1887

Abstract

A novel xylan-degrading actinomycete, strain YIM 61515, was isolated from surface-sterilized leaves of the medicinal plant . Cells were Gram-positive and non-spore-forming, produced primary branches and formed white to yellowish white colonies on the media tested. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61515 was most similar to V54A and V45 (99.4 and 99.1 % sequence similarity, respectively). The isolate formed a separate lineage in a cluster containing V54A. 16S rRNA gene sequence similarity between the isolate and other members of the genus ranged from 96.3 to 98.4 %. Chemotaxonomic data, including major menaquinones, fatty acid compositions and polar lipid profiles, supported the placement of strain YIM 61515 in the genus . DNA–DNA relatedness, physiological and biochemical data showed that strain YIM 61515 could be distinguished from members of all known species of the genus and therefore represented a novel species. The name sp. nov. is proposed, with YIM 61515 ( = DSM 21603 = CCTCC AA 208046) as type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032185-0
2012-01-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/84.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032185-0&mimeType=html&fmt=ahah

References

  1. Alonso-Vega P., Santamaría R. I., Martínez-Molina E., Trujillo M. E.. ( 2008;). Promicromonospora kroppenstedtii sp. nov., isolated from sandy soil. . Int J Syst Evol Microbiol 58:, 1476–1481. [CrossRef][PubMed]
    [Google Scholar]
  2. Busse H.-J., Zlamala C., Buczolits S., Lubitz W., Kämpfer P., Takeuchi M.. ( 2003;). Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval ‘Virgilkapelle’ in Vienna. . Int J Syst Evol Microbiol 53:, 1503–1507. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–789. [CrossRef]
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  7. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef][PubMed]
    [Google Scholar]
  8. Hasegawa T., Takizawa M., Tanida S.. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  9. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J.. ( 2005;). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. . Int J Syst Evol Microbiol 55:, 1939–1944. [CrossRef][PubMed]
    [Google Scholar]
  10. Jiang L., Li M., Li W., Cui X., Xu L., Jiang C.. ( 2001;). [Study on the application of quantitative analysis of cell-wall amino acids in actinomycetes classification]. . Wei Sheng Wu Xue Bao 41:, 270–277 (in Chinese).[PubMed]
    [Google Scholar]
  11. Jiang Y., Wiese J., Cao Y.-R., Xu L.-H., Imhoff J. F., Jiang C.-L.. ( 2009;). Promicromonospora flava sp. nov., isolated from sediment of the Baltic Sea. . Int J Syst Evol Microbiol 59:, 1599–1602. [CrossRef][PubMed]
    [Google Scholar]
  12. Kalakoutskii L. V., Agre N. S., Prauser H., Evtushenko L. I.. ( 1989;). Genus Promicromonospora Krasil’nikov, Kalakoutskii and Kirillova 1961a, 107AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2392–2395. Edited by Williams S. T., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  13. Kelly K. L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  14. Kluge A. G., Farris J. S.. ( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  15. Krasil’nikov N. A., Kalakoutskii L. V., Kirillova N. F.. ( 1961;). A new genus of Actinomycetales, Promicromonospora gen. nov.. Bull Acad Sci USSR Ser Biol 1:, 107–112.
    [Google Scholar]
  16. Kumar S., Tamura K., Nei M.. ( 2004;). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef][PubMed]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 227–291. Edited by Dietz A., Thayer J... Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  18. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  19. Martin K., Schäfer J., Kämpfer P.. ( 2010;). Promicromonospora umidemergens sp. nov., isolated from moisture from indoor wall material. . Int J Syst Evol Microbiol 60:, 537–541. [CrossRef][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  22. Qin S., Wang H.-B., Chen H.-H., Zhang Y.-Q., Jiang C.-L., Xu L.-H., Li W.-J.. ( 2008;). Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. . Int J Syst Evol Microbiol 58:, 2525–2528. [CrossRef][PubMed]
    [Google Scholar]
  23. Qin S., Li J., Chen H. H., Zhao G. Z., Zhu W. Y., Jiang C. L., Xu L. H., Li W. J.. ( 2009;). Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. . Appl Environ Microbiol 75:, 6176–6186. [CrossRef][PubMed]
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  26. Takahashi Y., Tanaka Y., Iwai Y., Omura S.. ( 1987;). Promicromonospora sukumoe sp. nov., a new species of the Actinomycetales. . J Gen Appl Microbiol 33:, 507–519. [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  28. Waksman S. A.. ( 1967;). The Actinomycetes. A Summary of Current Knowledge. New York:: Ronald Press;.
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032185-0
Loading
/content/journal/ijsem/10.1099/ijs.0.032185-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error