1887

Abstract

A novel Gram-negative, non-spore-forming, strictly anaerobic, heterotrophic bacterium, strain TY, was isolated from salty pickle wastewater. Cells were rod-shaped with comb-like flagella, slightly curved and very variable in length. Optimal growth occurred at 28 °C and pH 6.5. Cells were resistant to up to 50 g NaCl l. Strain TY produced acid from glycerol, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propionic acids. Tests for acid phosphatase and naphthol-AS-BI-phosphohydrolase activities were positive. The major fatty acids were C DMA (18.7 %), C (15.4 %), anteiso-C (15.2 %), C (13.3 %) and summed feature 5 (Cω7 and/or C) (11.0 %). The DNA G+C content was 35.9 mol%. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain TY represented a novel species of the genus (sequence similarity to other members of the genus ranged from 93.2 to 94.8 %). Based on its phenotypic, genotypic and phylogenetic characteristics, strain TY is proposed to represent a novel species, named sp. nov. (type strain TY  = JCM 17499  = DSM 24661).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970002)
  • National Science & Technology Major Project (Award 2008ZX07101-006-3)
  • Environmental Science Research & Design Institute of Zhejiang Province
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2012-09-01
2021-04-16
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