1887

Abstract

A novel actinobacterial strain, designated P4-5, was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P4-5 were Gram-stain-positive, oxidase- and catalase-positive, motile, short rods and the strain produced creamy white colonies on trypticase soy agar. The isolate contained demethylmenaquinone 7 (DMK-7) as the predominant isoprenoid quinone, Cω9 and anteiso-C as major fatty acids, diphosphatidylglycerol, phosphatidylglycerol and several unknown lipids in the polar lipid profile, galactose, glucose, mannose, arabinose, xylose (trace) and rhamnose as cell-wall sugars, and -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain P4-5 was 74.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain P4-5 was related most closely to KV-614 and CP177-2 (98.4 and 98.2 % similarity, respectively) and that it formed a separate lineage in the genus . Combined phenotypic and DNA–DNA hybridization data supported the conclusion that strain P4-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P4-5 ( = KCTC 19427  = CECT 7603). An emended description of the genus is also provided.

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2012-03-01
2019-10-15
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