1887

Abstract

Two previously uncharacterized strains of Gram-reaction-positive, anaerobic, coccus-shaped bacteria, designated strains WAL 18896 and WAL 18898, were recovered from human wound specimens and characterized using phenotypic, chemotaxonomic and molecular taxonomic methods. Comparative 16S rRNA gene sequence analysis and chemotaxonomic and biochemical characteristics demonstrated that these organisms are genotypically and phenotypically distinct and represent previously unidentified sublines within the order in the phylum . Pairwise sequence analysis demonstrated that the novel organisms had 91.9 % sequence similarity to each other and were most closely related to members of the genus . The major long-chain fatty acids of both strains were C C, Cω9 and Cω6,9. Based on the phenotypic and phylogenetic findings, strains WAL 18896 ( = CCUG 56065  = ATCC BAA-1640) and WAL 18898 ( = CCUG 56067  = ATCC BAA-1638  = DSM 22616) represent two novel species, for which the names sp. nov. and sp. nov. are proposed, respectively.

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2012-10-01
2019-10-22
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References

  1. Benson D. A., Boguski M. S., Lipman D. J., Ostell J.. ( 1997;). GenBank. . Nucleic Acids Res 25:, 1–6. [CrossRef][PubMed]
    [Google Scholar]
  2. Ezaki T., Kawamura Y., Li N., Li Z. Y., Zhao L., Shu S.. ( 2001;). Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus. . Int J Syst Evol Microbiol 51:, 1521–1528.[PubMed]
    [Google Scholar]
  3. Finegold S. M., Song Y., Liu C.. ( 2002;). Taxonomy–general comments and update on taxonomy of clostridia and anaerobic cocci. . Anaerobe 8:, 283–285. [CrossRef][PubMed]
    [Google Scholar]
  4. Fontana C., Favaro M., Pelliccioni M., Pistoia E. S., Favalli C.. ( 2005;). Use of the MicroSeq 500 16S rRNA gene-based sequencing for identification of bacterial isolates that commercial automated systems failed to identify correctly. . J Clin Microbiol 43:, 615–619. [CrossRef][PubMed]
    [Google Scholar]
  5. Garrity G. M., Bell J. A., Lilburn T.. ( 2005;). The revised road map to the manual. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol 2A, pp. 159–187. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;. [CrossRef]
    [Google Scholar]
  6. Jousimies-Somer H., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M.. ( 2002;). Wadsworth-KTL Anaerobic Bacteriology Manual, , 6th edn.. Belmont, CA:: Star Publishing;.
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  9. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  10. Murdoch D. A.. ( 1998;). Gram-positive anaerobic cocci. . Clin Microbiol Rev 11:, 81–120.[PubMed]
    [Google Scholar]
  11. Murdoch D. A., Shah H. N.. ( 1999;). Reclassification of Peptostreptococcus magnus (Prevot 1933) Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov.. Anaerobe 5:, 555–559. [CrossRef]
    [Google Scholar]
  12. O’Hara C. M., Tenover F. C., Miller J. M.. ( 1993;). Parallel comparison of accuracy of API 20E, Vitek GNI, MicroScan Walk/Away Rapid ID, and Becton Dickinson Cobas Micro ID-E/NF for identification of members of the family Enterobacteriaceae and common Gram-negative, non-glucose-fermenting bacilli. . J Clin Microbiol 31:, 3165–3169.[PubMed]
    [Google Scholar]
  13. Rooney A. P., Swezey J. L., Pukall R., Schumann P., Spring S.. ( 2011;). Peptoniphilus methioninivorax sp. nov., a Gram-positive anaerobic coccus isolated from retail ground beef. . Int J Syst Evol Microbiol 61:, 1962–1967. [CrossRef][PubMed]
    [Google Scholar]
  14. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . Newark, DE:: MIDI, Inc.;
  15. Song Y., Liu C., McTeague M., Finegold S. M.. ( 2003;). 16S ribosomal DNA sequence-based analysis of clinically significant Gram-positive anaerobic cocci. . J Clin Microbiol 41:, 1363–1369. [CrossRef][PubMed]
    [Google Scholar]
  16. Song Y., Liu C., Finegold S. M.. ( 2007a;). Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. isolated from clinical specimens of human origin. . J Clin Microbiol 45:, 1746–1752. [CrossRef][PubMed]
    [Google Scholar]
  17. Song Y., Liu C., Finegold S. M.. ( 2007b;). Development of a flow chart for identification of Gram-positive anaerobic cocci in the clinical laboratory. . J Clin Microbiol 45:, 512–516. [CrossRef][PubMed]
    [Google Scholar]
  18. Stackebrandt E., Ebers J.. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  20. Ŝvec P., Devriese L. A.. ( 2009;). Family IV. Enterococcaceae fam. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol 2, pp. 594–607. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F.A., Schleifer K.-H, Whitman W. B. .. New York:: Springer;.
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity for progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  23. Tindall B. J., Euzéby J. P.. ( 2006;). Proposal of Parvimonas gen. nov. and Quatrionicoccus gen. nov. as replacements for the illegitimate, prokaryotic, generic names Micromonas Murdoch and Shah 2000 and Quadricoccus Maszenan et al. 2002, respectively. . Int J Syst Evol Microbiol 56:, 2711–2713. [CrossRef][PubMed]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
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