%0 Journal Article %A Zhao, Guo-Zhen %A Li, Jie %A Zhu, Wen-Yong %A Tian, Shou-Zheng %A Zhao, Li-Xing %A Yang, Ling-Ling %A Xu, Li-Hua %A Li, Wen-Jun %T Rhodococcus artemisiae sp. nov., an endophytic actinobacterium isolated from the pharmaceutical plant Artemisia annua L. %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_4 %P 900-905 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.031930-0 %I Microbiology Society, %X A Gram-positive, non-motile actinobacterium, designated YIM 65754T, was isolated from the stem of Artemisia annua L., collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 65754T comprised an evolutionary lineage within the genus Rhodococcus . The isolate clustered with Rhodococcus pyridinivorans PDB9T, Rhodococcus gordoniae W 4937T and Rhodococcus rhodochrous DSM 43241T, with which it shared 98.4, 97.9 and 97.8 % 16S rRNA gene sequence similarities, respectively. However, DNA–DNA relatedness demonstrated that strain YIM 65754T was distinct from its closest phylogenetic neighbours. The cell-wall peptidoglycan contained meso-diaminopimelic acid, arabinose, galactose, mannose and glucose (cell-wall chemotype IV). The major menaquinone was MK-8(H2) and the predominant fatty acids were C16 : 0 (27.83 %), iso-C15 : 0 2-OH and/or C16 : 1ω7c (20.21 %) and 10-methyl C18 : 0 (17.50 %). The DNA G+C content was 66.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, the isolate represents a novel species of the genus Rhodococcus , for which the name Rhodococcus artemisiae sp. nov. is proposed; the type strain is YIM 65754T ( = CCTCC AA 209042T = DSM 45380T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.031930-0