1887

Abstract

A Gram-positive, non-motile actinobacterium, designated YIM 65754, was isolated from the stem of L., collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 65754 comprised an evolutionary lineage within the genus . The isolate clustered with PDB9, W 4937 and DSM 43241, with which it shared 98.4, 97.9 and 97.8 % 16S rRNA gene sequence similarities, respectively. However, DNA–DNA relatedness demonstrated that strain YIM 65754 was distinct from its closest phylogenetic neighbours. The cell-wall peptidoglycan contained -diaminopimelic acid, arabinose, galactose, mannose and glucose (cell-wall chemotype IV). The major menaquinone was MK-8(H) and the predominant fatty acids were C (27.83 %), iso-C 2-OH and/or Cω7 (20.21 %) and 10-methyl C (17.50 %). The DNA G+C content was 66.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, the isolate represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is YIM 65754 ( = CCTCC AA 209042 = DSM 45380).

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2012-04-01
2019-10-23
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