1887

Abstract

An aerobic, pink-pigmented, chemo-organotrophic bacterium, designated strain SN10, was isolated from a methanotrophic enrichment culture obtained from an acidic peat. This isolate was represented by Gram-negative, non-motile rods that multiply by normal cell division and form rosettes. Strain SN10 is an obligately acidophilic, mesophilic bacterium capable of growth at pH 3.2–6.6 (with an optimum at pH 4.7–5.2) and at 6–32 °C (with an optimum at 20–24 °C). The preferred growth substrates are sugars and several heteropolysaccharides of plant and microbial origin, such as pectin, lichenan, fucoidan and gellan gum. While not being capable of growth on C compounds, strain SN10 can develop in co-culture with exopolysaccharide-producing methanotrophs by utilization of their capsular material. The major fatty acids determined in strain SN10 using the conventional lipid extraction procedure are iso-C and Cω7. Upon hydrolysis of total cell material, substantial amounts of the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (isodiabolic acid) were also detected. The polar lipids are two phosphohexoses, phosphatidylethanolamine, phosphatidylglycerol and several phospholipids of unknown structure. The major quinone is MK-8. Pigments are carotenoids. The G+C content of the DNA is 60.7 mol%. Strain SN10 forms a separate lineage within subdivision 1 of the phylum and displays 94.0–95.4 % 16S rRNA gene sequence similarity to members of the genera and , 93.0–93.7 % similarity to members of the genus and 92.2–92.3 % similarity to the type strains of and . Therefore, strain SN10 is classified within a novel genus and species, for which the name gen. nov., sp. nov. is proposed. Strain SN10 ( = LMG 25276  = DSM 22489) is the type strain of . An emended description of Koch 2008 is also given.

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2012-03-01
2019-10-18
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