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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_9

sp. nov., sp. nov., sp. nov. and sp. nov., novel acidobacteria from tundra soil Free

Abstract

Four aerobic bacteria, designated MP5ACTX2, MP5ACTX8, MP5ACTX9 and S6CTX5A, were isolated from tundra soil of north-western Finland (69° 03′ N 20° 50′ E). Cells of all isolates were Gram-negative, non-motile rods. Phylogenetic analysis indicated that they belonged to the genus of subdivision 1 of the phylum . 16S rRNA gene sequence similarity between the new isolates and the type strains of , , and ranged from 94 to 99 %. Analysis of the RNA polymerase beta subunit () gene sequence indicated that the isolates represented novel species of the genus (<92 % sequence similarity between the isolates and members of the genus ). This was also confirmed by low DNA–DNA relatedness (31 %) between strain S6CTX5A and the type strain of , which exhibited 99.1 % 16S rRNA gene sequence similarity and 91.7 % gene sequence similarity. The isolates grew at pH 3.5–6.5 and at 4–26 °C. Sugars were the preferred growth substrates. The major cellular fatty acids were iso-C, Cω7 and C and the major isoprenoid quinone was MK-8. The DNA G+C content was 56–60 mol%. On the basis of phylogenetic analysis and chemotaxonomic and physiological data, the isolates represent four novel species of the genus , for which the names MP5ACTX2 ( = ATCC BAA-1858 = DSM 23128), MP5ACTX8 ( = ATCC BAA-1857 = DSM 23137), MP5ACTX9 (ATCC BAA-1859 = DSM 23138) and S6CTX5A ( = LMG 26174 = DSM 23136) are proposed. An emended description of the genus is also presented.

  • Published Online:
Funding
This study was supported by the:
  • Academy of Finland (Award 123725 and 106208)
  • National Science Foundation (Award IPY 0732956)
  • Lapland Regional Fund of the Finnish Cultural Foundation
© 2012 IUMS
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2012-09-01
2025-03-23
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Granulicella arctica sp. nov., Granulicella mallensis sp. nov., Granulicella tundricola sp. nov. and Granulicella sapmiensis sp. nov., novel acidobacteria from tundra soil
Int J Syst Evol Microbiol 62, 2097 (2012); https://doi.org/10.1099/ijs.0.031864-0
/content/journal/ijsem/10.1099/ijs.0.031864-0
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