RT Journal Article SR Electronic(1) A1 Huang, Hai-Yu A1 Li, Jie A1 Zhao, Guo-Zhen A1 Zhu, Wen-Yong A1 Yang, Ling-Ling A1 Tang, Hai-Yun A1 Xu, Li-Hua A1 Li, Wen-JunYR 2012 T1 Sphingomonas endophytica sp. nov., isolated from Artemisia annua L. JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_7 SP 1576 OP 1580 DO https://doi.org/10.1099/ijs.0.031484-0 PB Microbiology Society, SN 1466-5034, AB A novel bacterium (strain YIM 65583T) belonging to the genus Sphingomonas was isolated from surface-sterilized tissue of Artemisia annua L., which was collected from Yunnan province, south-west China. Its morphology, physiology and biochemical features were consistent with those of members of the genus Sphingomonas . Analysis of the 16S rRNA gene sequence of strain YIM 65583T further confirmed that it should be classified as a member of the genus Sphingomonas and was most closely related to Sphingomonas phyllosphaerae FA2T (99.7 %) and Sphingomonas yunnanensis YIM 003T (98.3 %). The isolate was Gram-negative and formed yellow-pigmented colonies on ISP 2 medium. It grew optimally at pH 6.0–8.0, at 20–37 °C and in the presence of 0–1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-10; C18 : 1ω7c, anteiso-C16 : 1, C14 : 0-2OH, C17 : 1ω6c, C16 : 0 and C15 : 0 were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.3 mol%. The DNA–DNA relatedness values of the isolate YIM 65583T with S. phyllosphaerae FA2T and S. yunnanensis YIM 003T were 43.1 % and 37.9 %, respectively. Based on these features, it is concluded that the strain represents a novel species of the genus Sphingomonas, for which the name Sphingomonas endophytica sp. nov. is proposed, with YIM 65583T ( = CCTCC AA 209035T = JCM 17394T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.031484-0