1887

Abstract

A novel bacterium (strain YIM 65583) belonging to the genus was isolated from surface-sterilized tissue of L., which was collected from Yunnan province, south-west China. Its morphology, physiology and biochemical features were consistent with those of members of the genus . Analysis of the 16S rRNA gene sequence of strain YIM 65583 further confirmed that it should be classified as a member of the genus and was most closely related to FA2 (99.7 %) and YIM 003 (98.3 %). The isolate was Gram-negative and formed yellow-pigmented colonies on ISP 2 medium. It grew optimally at pH 6.0–8.0, at 20–37 °C and in the presence of 0–1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-10; Cω7, anteiso-C, C-2OH, Cω6, C and C were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.3 mol%. The DNA–DNA relatedness values of the isolate YIM 65583 with FA2 and YIM 003 were 43.1 % and 37.9 %, respectively. Based on these features, it is concluded that the strain represents a novel species of the genus s, for which the name sp. nov. is proposed, with YIM 65583 ( = CCTCC AA 209035 = JCM 17394) as the type strain.

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2012-07-01
2024-12-03
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