1887

Abstract

Two coccoid, non-motile bacteria were isolated from seawater in the north-western Pacific Ocean near Japan. The two strains, designated S1-36 and S1-72, were Gram-negative, obligately aerobic, heterotrophic and catalase-negative. They were able to reduce nitrate to nitrogen. Both strains required NaCl for growth, with optimum growth in 2 % NaCl, and grew at 15–30 °C, with optimum growth at 20–25 °C. Genomic DNA G+C contents of strains S1-36 and S1-72 were 59.6 and 59.4 mol%, respectively. The predominant isoprenoid quinone was Q-8 and major cellular fatty acids were Cω7, Cω7 and Cω8. Analyses of 16S rRNA gene sequences revealed that strains S1-36 and S1-72 were related to each other (96.1 % sequence similarity) and both strains showed 92.3–94.7 % sequence similarity with members of the genus . On the basis of phenotypic and phylogenetic features, strains S1-36 and S1-72 should be classified as representatives of two novel species in a new genus, gen. nov., within the class . The names proposed are sp. nov., the type species of the genus, with S1-36 ( = NBRC 107739 = KCTC 23429) as type strain, and sp. nov., with S1-72 ( = NBRC 107742 = KCTC 23430) as type strain

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.031443-0
2012-08-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1784.html?itemId=/content/journal/ijsem/10.1099/ijs.0.031443-0&mimeType=html&fmt=ahah

References

  1. Dittmer J. C. , Lester R. L. . ( 1964; ). A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res 15:, 126–127.[PubMed]
    [Google Scholar]
  2. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Graeber I. , Kaesler I. , Borchert M. S. , Dieckmann R. , Pape T. , Lurz R. , Nielsen P. , von Döhren H. , Michaelis W. , Szewzyk U. . ( 2008; ). Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1. . Int J Syst Evol Microbiol 58:, 585–590. [CrossRef] [PubMed]
    [Google Scholar]
  5. Guindon S. , Gascuel O. . ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hwang C. Y. , Cho B. C. . ( 2009; ). Spongiibacter tropicus sp. nov., isolated from a Synechococcus culture. . Int J Syst Evol Microbiol 59:, 2176–2179. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  8. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  9. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–147. Edited by Stackebrandt E. , Goodfellow M. . . New York:: John Wiley and Sons;.
    [Google Scholar]
  10. Lee Y. K. , Hong S. G. , Cho H. H. , Cho K. H. , Lee H. K. . ( 2007; ). Dasania marina gen. nov., sp. nov., of the order Pseudomonadales, isolated from Arctic marine sediment. . J Microbiol 45:, 505–509.[PubMed]
    [Google Scholar]
  11. Li H. , Zhang S. , Chen C. , Zhang Y. , Gao Z. , Yu Y. , Chen X. , Chen B. , Zhang Y. . ( 2011; ). Zhongshania antarctica gen. nov., sp. nov. and Zhongshania guokunii sp. nov., new members of the Gammaproteobacteria isolated from coastal attached (fast) ice and surface seawater of the Antarctic, respectively. . Int J Syst Evol Microbiol 61:, 2052–2057. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lucena T. , Pascual J. , Garay E. , Arahal D. R. , Macián M. C. , Pujalte M. J. . ( 2010; ). Haliea mediterranea sp. nov., a marine gammaproteobacterium. . Int J Syst Evol Microbiol 60:, 1844–1848. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  14. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  15. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  16. Murray R. G. E. , Doetsch R. N. , Robinow F. . ( 1994; ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Park S. , Yoshizawa S. , Kogure K. , Yokota A. . ( 2011; ). Oceanicoccus sagamiensis gen. nov., sp. nov., a gammaproteobacterium isolated from sea water of Sagami Bay in Japan. . J Microbiol 49:, 233–237. [CrossRef] [PubMed]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  20. Spring S. , Lünsdorf H. , Fuchs B. M. , Tindall B. J. . ( 2009; ). The photosynthetic apparatus and its regulation in the aerobic gammaproteobacterium Congregibacter litoralis gen. nov., sp. nov.. PLoS ONE 4:, e4866. [CrossRef] [PubMed]
    [Google Scholar]
  21. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  22. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  23. Urios L. , Agogué H. , Intertaglia L. , Lesongeur F. , Lebaron P. . ( 2008a; ). Melitea salexigens gen. nov., sp. nov., a gammaproteobacterium from the Mediterranean Sea. . Int J Syst Evol Microbiol 58:, 2479–2483. [CrossRef] [PubMed]
    [Google Scholar]
  24. Urios L. , Intertaglia L. , Lesongeur F. , Lebaron P. . ( 2008b; ). Haliea salexigens gen. nov., sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. . Int J Syst Evol Microbiol 58:, 1233–1237. [CrossRef] [PubMed]
    [Google Scholar]
  25. Urios L. , Intertaglia L. , Lesongeur F. , Lebaron P. . ( 2009; ). Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. . Int J Syst Evol Microbiol 59:, 1188–1192. [CrossRef] [PubMed]
    [Google Scholar]
  26. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  27. Worliczek H. L. , Kämpfer P. , Rosengarten R. , Tindall B. J. , Busse H. J. . ( 2007; ). Polar lipid and fatty acid profiles – re-vitalizing old approaches as a modern tool for the classification of mycoplasmas?. Syst Appl Microbiol 30:, 355–370. [CrossRef] [PubMed]
    [Google Scholar]
  28. Xie C. H. , Yokota A. . ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yan S. , Fuchs B. M. , Lenk S. , Harder J. , Wulf J. , Jiao N.-Z. , Amann R. . ( 2009; ). Biogeography and phylogeny of the NOR5/OM60 clade of Gammaproteobacteria . . Syst Appl Microbiol 32:, 124–139. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.031443-0
Loading
/content/journal/ijsem/10.1099/ijs.0.031443-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error