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Abstract

A novel red-pigmented halophilic archaeon, strain EB27, was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain EB27 were non-motile and pleomorphic (rods to triangular or disc-shaped). Strain EB27 required at least 2.5 M NaCl and 0.1 M MgCl for growth. Optimal growth was achieved at 4 M NaCl and 0.5 M MgCl. The optimum pH and temperature for growth were pH 7.5 and 40 °C; it was able to grow at pH 6.0–8.0 and 25–50 °C. 16S rRNA gene sequence analysis showed that strain EB27 is a member of the family ; however, levels of 16S rRNA gene sequence similarity were as low as 90.0, 89.3 and 89.1 % to the most closely related haloarchaeal taxa, namely DS12, 2-9-1 and AX-2, respectively. The DNA GC content of strain EB27 was 61 mol%. Strain EB27 contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, together with two minor phospholipids. The only quinone present was MK-8(II-H). Physiological, biochemical and phylogenetic differences between strain EB27 and recognized genera of extremely halophilic archaea suggest that this strain represents a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of , the type species of the new genus, is strain EB27 ( = IBRC-M 10015 = CGMCC 1.11001).

Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-01)
  • International Foundation for Science (IFS) (Award A/4527-1)
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2012-06-01
2021-10-24
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