1887

Abstract

Two strains of dissimilatory iron-reducing bacteria, which could couple lactate oxidation to iron reduction for energy conservation, were isolated from Arctic marine sediment. The strains, IR12 and IR26, were both Gram-staining-negative, catalase- and oxidase-positive and facultative anaerobes. Their cells were rod-shaped and motile by means of a polar flagellum. Both strains grew in the presence of 0.5–3.5 % (w/v) NaCl, with an absolute requirement for Na. Both were psychrotolerant since they could grow at 4-28 °C but had an optimum growth temperature of 20 °C. Both grew at pH 4.5–9.0 (optimum, pH 7.5). The major fatty acids of strains IR12 and IR26 were summed feature 3 (Cω6 and/or Cω7) and C. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains IR12 and IR26 belonged to the class and were most closely related to M7, NF22 and ACAM 591 (with 98.5 and 98.8 %, 98.5 and 98.8 %, and 98.5 and 98.8 % sequence similarities, respectively). The genomic DNA G+C contents of strains IR12 and IR26 were 40.0 and 40.3 mol%, respectively. DNA–DNA relatedness data indicated that the two novel strains represented a single species that was distinct from M7, NF22 and ACAM 591. Based on the phylogenetic, phenotypic and DNA–DNA relatedness data, the two new strains represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is IR12 ( = KCTC 23109 = JCM 16723).

Funding
This study was supported by the:
  • National Research Foundation (Award 2010-0014384)
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2012-05-01
2021-10-26
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