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Abstract

A Gram-stain-negative, rod-shaped bacterium, designated strain Y9, was isolated from a soil sample collected in Ningxia Province in China and was characterized to determine its taxonomic position. Strain Y9 contained Q-8 as the predominant ubiquinone. Major fatty acid components were summed feature 3 (Cω7 and/or iso-C 2-OH) and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain Y9 was 68.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus Levels of 16S rRNA gene sequence similarity between strain Y9 and the type strains of recognized species ranged from 95.2 to 98.2 %, the highest values being with 45 (98.2 %) and 101 (98.0 %). However, levels of DNA–DNA relatedness between strain Y9 and KCTC 12343 and KCTC 12345 were 37 and 26 %, respectively. Strain Y9 was clearly differentiated from its nearest phylogenetic relatives in the genus based on phenotypic, chemotaxonomic and phylogenetic properties. Therefore, strain Y9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y9 ( = CGMCC 1.10685  = KCTC 23585).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970009 and 31070002)
  • Special Fund for Public Welfare Industrial (Agriculture) Research of China (Award 200903001)
  • Basic Research Foundation of Beijing Institute of Technology (Award 20091642006)
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2012-03-01
2021-10-15
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