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Abstract
A Gram-stain-negative, rod-shaped bacterium, designated strain Y9T, was isolated from a soil sample collected in Ningxia Province in China and was characterized to determine its taxonomic position. Strain Y9T contained Q-8 as the predominant ubiquinone. Major fatty acid components were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain Y9T was 68.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus Massilia. Levels of 16S rRNA gene sequence similarity between strain Y9T and the type strains of recognized Massilia species ranged from 95.2 to 98.2 %, the highest values being with Massilia albidiflava 45T (98.2 %) and Massilia lutea 101T (98.0 %). However, levels of DNA–DNA relatedness between strain Y9T and M. albidiflava KCTC 12343T and M. lutea KCTC 12345T were 37 and 26 %, respectively. Strain Y9T was clearly differentiated from its nearest phylogenetic relatives in the genus Massilia based on phenotypic, chemotaxonomic and phylogenetic properties. Therefore, strain Y9T is considered to represent a novel species of the genus Massilia, for which the name Massilia flava sp. nov. is proposed. The type strain is Y9T ( = CGMCC 1.10685T = KCTC 23585T).
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Funding
- National Natural Science Foundation of China (Award 30970009 and 31070002)
- Special Fund for Public Welfare Industrial (Agriculture) Research of China (Award 200903001)
- Basic Research Foundation of Beijing Institute of Technology (Award 20091642006)