1887

Abstract

A facultatively anaerobic, chitinolytic bacterium, strain KL-9, was isolated from a freshwater lake in Taiwan and characterized by using a polyphasic taxonomic approach. Cells of strain KL-9 were Gram-negative, rod-shaped, motile by means of a single polar flagellum and non-spore-forming. Growth occurred at 15–40 °C (optimum, 30–37 °C), at pH 7.0–9.0 (optimum, pH 8.0) and with 0–1.0 % NaCl (optimum, 0 %). The predominant fatty acids were summed feature 3 (comprising Cω7 and/or Cω6) and C. The major isoprenoid quinone was Q-8. The DNA G+C content of strain KL-9 was 64.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine and several uncharacterized phospholipids and aminolipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KL-9 formed a distinct lineage with respect to closely related genera within the class , being most closely related to members of the genera , , and . Levels of 16S rRNA gene sequence similarity with respect to the type strains of type species of these genera were below 91 %. On the basis of genotypic and phenotypic data, strain KL-9 is thus considered to represent a novel species of a new genus within the class , for which the name gen. nov., sp. nov. is proposed. The type strain of is KL-9 ( = BCRC 80168 = LMG 25530).

Funding
This study was supported by the:
  • , National Science Council, Taiwan, Republic of China , (Award NSC 99-2621-B-022-001-MY3)
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2012-05-01
2020-12-01
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