1887

Abstract

A non-motile and non-endospore-forming rod, strain NBRC 16403, was isolated from the phyllosphere of a sedge ( sp.). 16S rRNA gene sequence analysis indicated that strain NBRC 16403 was closely related to DSM 19813 (98.6 % 16S rRNA gene sequence similarity), wged11 (97.8 %) and RB-62 (97.8 %). The peptidoglycan (B2γ type) contained - and -2,4-diaminobutyric acids, -alanine, glycine and -3-hydroxyglutamic acid, which replaced glutamic acid almost completely. The predominant menaquinones were MK-10 and MK-11. The polar lipid pattern comprised diphosphatidylglycerol, phosphatidylglycerol, three glycolipids and minor amounts of other polar lipids. The major fatty acids were anteiso-C, iso-C and anteiso-C; no cyclohexyl-C was detected. The DNA G+C content was 71.0 mol%. The results of phylogenetic and DNA–DNA relatedness studies, along with phenotypic differences between strain NBRC 16403 and recognized members of the genus , indicated that the isolate should be assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NBRC 16403 ( = VKM Ac-2058).

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2012-04-01
2019-10-20
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