1887

Abstract

Four rhizobial strains representing a previously defined novel group in the genus and isolated from species in China were further characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus , with LMG 11892 as the closest neighbour sharing a sequence similarity of 99.8 %. Comparative sequence analysis of the , , , , and genes, SDS-PAGE of whole-cell soluble proteins, DNA–DNA hybridization, fatty acid profiles and a series of phenotypic and physiological tests differentitated the novel group from all recognized species of the genus . Based on the data obtained in the present and previous studies, this group represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CCBAU 01550 ( = HAMBI 3029 = LMG 24822), and could form effective nodules on , and , and ineffective nodules on in cross-nodulation tests.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970004 and 30870004)
  • Chinese Universities Scientific Fund (Award 2009JD19)
  • National Basic Research Program of China (Award 2010CB126500)
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2012-09-01
2021-04-17
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