1887

Abstract

Strains LMG 8159 and LMG 11499 were reclassified by a polyphasic approach, including 16S rRNA gene sequence analysis, 16S–23S rRNA intergenic spacer (IGS) sequence analysis, (GTG)-PCR fingerprinting, RAPD fingerprinting, fatty acid methyl ester analysis and an analysis of phenotypic features using API 50 CH. The two strains were closely related to the type strains of the three defined subspecies of , showing 99.7–99.9 % 16S rRNA gene sequence similarity, 99.2 % 16S–23S rRNA gene intergenic spacer sequence similarity, 97.1–97.4 % gene sequence similarity and 98.0–98.2 % gene sequence similarity. Low gene sequence similarity (91.4–91.7 %), (GTG)-PCR fingerprinting, RAPD fingerprinting, fatty acid compositions and phenotypic features allowed us to differentiate strains LMG 8159 and LMG 11499 from all established subspecies within . Based upon the data obtained in the present and previous studies, a novel subspecies is proposed within the species , subsp. subsp. nov., with the type strain LMG 8159 ( = ATCC 9135  = DSM 20241  = NCIMB 6992).

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2012-07-01
2020-01-19
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