1887

Abstract

Strains LMG 8159 and LMG 11499 were reclassified by a polyphasic approach, including 16S rRNA gene sequence analysis, 16S–23S rRNA intergenic spacer (IGS) sequence analysis, (GTG)-PCR fingerprinting, RAPD fingerprinting, fatty acid methyl ester analysis and an analysis of phenotypic features using API 50 CH. The two strains were closely related to the type strains of the three defined subspecies of , showing 99.7–99.9 % 16S rRNA gene sequence similarity, 99.2 % 16S–23S rRNA gene intergenic spacer sequence similarity, 97.1–97.4 % gene sequence similarity and 98.0–98.2 % gene sequence similarity. Low gene sequence similarity (91.4–91.7 %), (GTG)-PCR fingerprinting, RAPD fingerprinting, fatty acid compositions and phenotypic features allowed us to differentiate strains LMG 8159 and LMG 11499 from all established subspecies within . Based upon the data obtained in the present and previous studies, a novel subspecies is proposed within the species , subsp. subsp. nov., with the type strain LMG 8159 ( = ATCC 9135  = DSM 20241  = NCIMB 6992).

Funding
This study was supported by the:
  • Program for Changjiang Scholars and Innovative Research Team in University (Award IRT0959)
  • Science Foundation of North-east Agricultural University of China
  • National Natural Science Foundation of China (Award 31101342)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.031203-0
2012-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1548.html?itemId=/content/journal/ijsem/10.1099/ijs.0.031203-0&mimeType=html&fmt=ahah

References

  1. Akopyanz N., Bukanov N. O., Westblom T. U., Kresovich S., Berg D. E. 1992; DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 20:5137–5142 [View Article][PubMed]
    [Google Scholar]
  2. An D., Cai S., Dong X. 2006; Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 56:2043–2048 [View Article][PubMed]
    [Google Scholar]
  3. De Bruyne K., Schillinger U., Caroline L., Boehringer B., Cleenwerck I., Vancanneyt M., De Vuyst L., Franz C. M. A. P., Vandamme P. 2007; Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species. Int J Syst Evol Microbiol 57:2952–2959 [View Article][PubMed]
    [Google Scholar]
  4. Ehrmann M. A., Freiding S., Vogel R. F. 2009; Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine. Int J Syst Evol Microbiol 59:943–947 [View Article][PubMed]
    [Google Scholar]
  5. Farrow J. A. E., Facklam R. R., Collins M. D. 1989; Nucleic acid homologies of some vancomycin-resistant leuconostocs and description of Leuconostoc citreum sp. nov. and Leuconostoc pseudomesenteroides sp. nov.. Int J Syst Bacteriol 39:279–283 [View Article]
    [Google Scholar]
  6. Garvie E. I. 1976; Hybridization between the deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria. Int J Syst Bacteriol 26:116–122 [View Article]
    [Google Scholar]
  7. Garvie E. I. 1983; Leuconostoc mesenteroides subsp. cremoris (Knudsen and Sørensen) comb. nov. and Leuconostoc mesenteroides subsp. dextranicum (Beijerinck) comb. nov.. Int J Syst Bacteriol 33:118–119 [View Article]
    [Google Scholar]
  8. Hontebeyrie M., Gasser F. 1977; Deoxyribonucleic acid homologies in the genus Leuconostoc . Int J Syst Bacteriol 27:9–14 [View Article]
    [Google Scholar]
  9. Rachman C. N., Kabadjova P., Prévost H., Dousset X. 2003; Identification of Lactobacillus alimentarius and Lactobacillus farciminis with 16S-23S rDNA intergenic spacer region polymorphism and PCR amplification using species-specific oligonucleotide. J Appl Microbiol 95:1207–1216 [View Article][PubMed]
    [Google Scholar]
  10. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc..
    [Google Scholar]
  11. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy. The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  12. Švec P., Nováková D., Zácková L., Kukletová M., Sedlácek I. 2008; Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans . Antonie van Leeuwenhoek 94:573–579 [View Article][PubMed]
    [Google Scholar]
  13. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  14. Torriani S., Zapparoli G., Dellaglio F. 1999; Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis . Appl Environ Microbiol 65:4351–4356[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.031203-0
Loading
/content/journal/ijsem/10.1099/ijs.0.031203-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error