1887

Abstract

Yeast strains were isolated from Vin Santo of Gambellara, a sweet white wine with the specificity of Controlled Designation of Origin produced from off-vine overripened grapes in the Veneto region (Italy). Comparative sequence analysis of the 26S rRNA gene revealed that three representative strains (ZO03-5, CA06-8 and ME06-9) constitute a taxon related to, but distinct from, . Similarity between the 26S rRNA gene domain D1/D2 sequence of the three isolates and was 97.9 %; moreover, the morphological characteristics and the physiological behaviour also supported recognition of a novel taxon of osmophilic non-psychrophilic yeast showing a flower-like arrangement of budding cells that remain attached to each other. The name is proposed for the novel species, with ZO03-5 ( = CBS 12191 = MUCL 53393) as the type strain.

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2011-12-01
2019-10-18
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References

  1. Beuchat L. R.. ( 1993;). Selective media for detecting and enumerating foodborne yeasts. . Int J Food Microbiol 19:, 1–14. [CrossRef][PubMed]
    [Google Scholar]
  2. Ciani M., Comitini F., Mannazzu I., Domizio P.. ( 2010;). Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking. . FEM Yeast Res 10:, 123–133. [CrossRef][PubMed]
    [Google Scholar]
  3. Cocolin L., Bisson L. F., Mills D. A.. ( 2000;). Direct profiling of the yeast dynamics in wine fermentations. . FEMS Microbiol Lett 189:, 81–87. [CrossRef][PubMed]
    [Google Scholar]
  4. Donèche B. J.. ( 1993;). Botrytized wines. . In Wine Microbiology and Biotechnology, pp. 327–351. Edited by Fleet G. H... Chur, Switzerland:: Harwood Academic Publishers;.
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Fleet G. H.. ( 1998;). The microbiology of alcoholic beverages. . In Microbiology of Fermented Foods, , 2nd edn., vol. 1, pp. 217–262. Edited by Wood B. J. B... London:: Blackie Academic & Professional;. [CrossRef]
    [Google Scholar]
  7. Fleet G. H., Lafon-Lafourcade S., Ribéreau-Gayon P.. ( 1984;). Evolution of yeasts and lactic Acid bacteria during fermentation and storage of bordeaux wines. . Appl Environ Microbiol 48:, 1034–1038.[PubMed]
    [Google Scholar]
  8. Fugelsang K. C.. ( 1996;). Zygosaccharomyces bailii: a spoilage yeast isolated from wine. . Technical bulletin 960804. Fresno, CA:: California Agricultural Technology Institute;.
  9. Gazzetta Ufficiale della Repubblica Italiana ( 1970;). Decreto del Presidente della Repubblica, 26/03/1970. Riconoscimento della denominazione di origine controllata del vino «Gambellara» ed approvazione del relativo disciplinare di produzione, no. 132 (29/5/1970), pp. 3435–3438.
  10. Jolly N. P., Augustyn O. P. H., Pretorius I. S.. ( 2006;). The role and use of non-Saccharomyces yeasts in wine production. . S Afr J Enol Vitic 27:, 15–39.
    [Google Scholar]
  11. Kämpfer P., Buczolits S., Albrecht A., Busse H.-J., Stackebrandt E.. ( 2003;). Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov.. Int J Syst Evol Microbiol 53:, 893–896. [CrossRef][PubMed]
    [Google Scholar]
  12. Kurtzman C. P.. ( 2003;). Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. . FEM Yeast Res 4:, 233–245. [CrossRef][PubMed]
    [Google Scholar]
  13. Kurtzman C. P.. ( 2011;). Phylogeny of the ascomycetous yeasts and the renaming of Pichia anomala to Wickerhamomyces anomalus. . Antonie van Leeuwenhoek 99:, 13–23. [CrossRef][PubMed]
    [Google Scholar]
  14. Kurtzman C. P., Fell J. W.. (editors) ( 1998;). The Yeasts: a Taxonomic Study, , 4th edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  15. Kurtzman C. P., Robnett C. J.. ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef][PubMed]
    [Google Scholar]
  16. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  17. Mills D. A., Johannsen E. A., Cocolin L.. ( 2002;). Yeast diversity and persistence in botrytis-affected wine fermentations. . Appl Environ Microbiol 68:, 4884–4893. [CrossRef][PubMed]
    [Google Scholar]
  18. Nei M., Kumar S.. ( 2000;). Molecular Evolution and Phylogenetics. New York, USA:: Oxford University Press;.
    [Google Scholar]
  19. Parapouli M., Hatziloukas E., Drainas C., Perisynakis A.. ( 2010;). The effect of Debina grapevine indigenous yeast strains of Metschnikowia and Saccharomyces on wine flavour. . J Ind Microbiol Biotechnol 37:, 85–93. [CrossRef][PubMed]
    [Google Scholar]
  20. Ribéreau-Gayon P., Dubourdieu D., Donèche B., Lonvaud A.. ( 2006;). Cytology, taxonomy and ecology of grape and wine yeasts. . In Handbook of Enology. The Microbiology of Wine and Vinifications, vol. 1, pp. 1–51. Edited by Ribéreau-Gayon P., Dubourdieu D., Donèche B., Lonvaud A... Chichester, UK:: Wiley;. [CrossRef]
    [Google Scholar]
  21. Rosa C. A., Lachance M.-A.. ( 2005;). Zygosaccharomyces machadoi sp. n., a yeast species isolated from a nest of the stingless bee Tetragonisca angustula. . Lundiana 6: (Suppl.), 27–29.
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Steels H., Bond C. J., Collins M. D., Roberts I. N., Stratford M., James S. A.. ( 1999;). Zygosaccharomyces lentus sp. nov., a new member of the yeast genus Zygosaccharomyces Barker. . Int J Syst Bacteriol 49:, 319–327. [CrossRef][PubMed]
    [Google Scholar]
  24. Stratford M.. ( 2006;). Food and beverage spoilage yeasts. . In The Yeast Handbook. Yeasts in Food and Beverages, vol. 2, pp. 335–379. Edited by Querol A., Fleet G... Germany:: Springer-Verlag;. [CrossRef]
    [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  26. Tofalo R., Chaves-López C., Di Fabio F., Schirone M., Felis G. E., Torriani S., Paparella A., Suzzi G.. ( 2009;). Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must. . Int J Food Microbiol 130:, 179–187. [CrossRef][PubMed]
    [Google Scholar]
  27. Yarrow D.. ( 1998;). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77–100. Edited by Kurtzman C. P., Fell J. W... Amsterdam:: Elsevier Science Publishers;. [CrossRef]
    [Google Scholar]
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vol. , part 12, pp. 3084 - 3088

Dendrogram obtained from analysis of M13 RAPD-PCR profiles for the 25 isolates analysed.

Growth of ZO03-5 in two different media.

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