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Volume 62, Issue Pt_4

gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond Free

Abstract

A novel halophilic, filamentous, actinomycete strain, designated CXB832, was isolated from a salt pond in Qingdao, China. Optimal growth occurred at 37 °C, pH 7.0–8.0 and 9–12 % (w/v) NaCl. Strain CXB832 formed pale yellow to deep yellow branched substrate mycelium without fragmentation. Abundant white aerial mycelium differentiated into long chains of spores and the spores were rod-shaped with smooth surfaces. Strain CXB832 contained -diaminopimelic acid as the diagnostic diamino acid of the cell-wall peptidoglycan, and glucose and xylose as the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipids, glycolipid and unidentified lipids. MK-10(H), MK-9(H), MK-10(H) and MK-10(H) were the predominant menaquinones. The major fatty acids were i-C (30.71 %), ai-C (13.31 %) and C (11.28 %). The G+C content of the DNA was 60.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family , but formed a separate lineage. The highest sequence similarities were to DSM 45083 (95.4 %) and DSM 45015 (94.9 %). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB832 represents a new genus and novel species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is CXB832 ( = DSM 45442 = LMG 25567).

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Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award No. 2007AA09Z434)
  • National Natural Science Foundation of China (Award No. 40876067)
© 2012 IUMS
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2012-04-01
2024-04-19
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Salinactinospora qingdaonensis gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond
Int J Syst Evol Microbiol 62, 954 (2012); https://doi.org/10.1099/ijs.0.031088-0
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