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Abstract

Strain MD5, an aerobic marine alphaproteobacterium, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized in a polyphasic study and was placed phylogenetically within the clade in the family . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MD5 is related to C02, T5, BS107 and TF-218, with 96.9, 96.2, 96.1 and 96.1 % sequence similarity, respectively. Phylogenetic analyses also showed that strain MD5 forms a stable clade only with C02. Strain MD5 requires Na plus a divalent cation (either Mg or Ca) to grow, does not reduce nitrate to nitrite and uses a large number of carbohydrates as sole carbon sources. It is positive for β-galactosidase and urease activities and aesculin hydrolysis. Enzyme activities displayed in the API ZYM strip were alkaline phosphatase, leucine arylamidase, acid phosphatase and α-glucosidase. Major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6; 70.9 %) and C (8.2 %). The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from the only described species of the genus . It is evident from the genotypic and phenotypic data obtained that the strain should be classified in a novel species in the genus . The name sp. nov. is proposed, with the type strain MD5 ( = CECT 7557  = KCTC 23350).

Funding
This study was supported by the:
  • , Spanish Ministerio de Educación y Ciencia , (Award CGL2010-18134 and CGL2005-02292)
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2012-04-01
2020-08-09
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