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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_4

sp. nov., an aerobic alphaproteobacterium isolated from surface seawater Free

Abstract

Strain MD5, an aerobic marine alphaproteobacterium, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized in a polyphasic study and was placed phylogenetically within the clade in the family . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MD5 is related to C02, T5, BS107 and TF-218, with 96.9, 96.2, 96.1 and 96.1 % sequence similarity, respectively. Phylogenetic analyses also showed that strain MD5 forms a stable clade only with C02. Strain MD5 requires Na plus a divalent cation (either Mg or Ca) to grow, does not reduce nitrate to nitrite and uses a large number of carbohydrates as sole carbon sources. It is positive for β-galactosidase and urease activities and aesculin hydrolysis. Enzyme activities displayed in the API ZYM strip were alkaline phosphatase, leucine arylamidase, acid phosphatase and α-glucosidase. Major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6; 70.9 %) and C (8.2 %). The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from the only described species of the genus . It is evident from the genotypic and phenotypic data obtained that the strain should be classified in a novel species in the genus . The name sp. nov. is proposed, with the type strain MD5 ( = CECT 7557  = KCTC 23350).

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Funding
This study was supported by the:
  • Spanish Ministerio de Educación y Ciencia (Award CGL2010-18134 and CGL2005-02292)
© 2012 IUMS
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2012-04-01
2025-03-22
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Tropicibacter multivorans sp. nov., an aerobic alphaproteobacterium isolated from surface seawater
Int J Syst Evol Microbiol 62, 844 (2012); https://doi.org/10.1099/ijs.0.030973-0
/content/journal/ijsem/10.1099/ijs.0.030973-0
/content/journal/ijsem/10.1099/ijs.0.030973-0
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