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Volume 63, Issue Pt_6

gen. nov., sp. nov., a new member of the family isolated from the South China Sea, and emended description of Free

Abstract

A taxonomic study was carried out on strain SCSWE24, isolated from a seawater sample collected from the South China Sea. Cells of strain SCSWE24 were Gram-negative, rod-shaped, non-motile, moderately halophilic and capable of reducing nitrate to nitrite. Growth was observed at salinities from 1.5 to 4.5 % and at 4–37 °C; it was unable to degrade gelatin. The dominant fatty acids (>15 %) were summed feature 3 (Cω7 and/or Cω6; 50.4 %) and C (21.1 %). The G+C content of the chromosomal DNA was 58.8 mol%. 16S rRNA gene sequence comparisons showed that strain SCSWE24 was most closely related to an uncultured bacterium clone Tun3b.F5 (98 %; GenBank accession no. FJ169216), and showed 92 % similarity to an endosymbiont bacterium from the bone-eating worm (clone Omu 9 c4791; FN773233). Levels of similarity between strain SCSWE24 and type strains of recognized species in the family were less than 93 %; the highest similarity was 92 %, to both JAMM 1866 and ‘’ PZ-5. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SCSWE24 formed a distinct evolutionary lineage within the family . Strain SCSWE24 was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain SCSWE24 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is SCSWE24 ( = CCTCC AB 209289  = LMG 25705  = MCCC 1F01088). An emended description of is also provided.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31070101, 30800033, 31070442 and 40930847)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201305041 and 201305016)
  • Program for Changjiang Scholars and Innovative Research Team in University (Award 41121091)
  • MEL Visiting Fellowship of State Key Laboratory of Marine Environmental Science, Xiamen University (Award MELRS1124)
© 2013 IUMS
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2013-06-01
2024-04-19
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Sinobacterium caligoides gen. nov., sp. nov., a new member of the family Oceanospirillaceae isolated from the South China Sea, and emended description of Amphritea japonica
Int J Syst Evol Microbiol 63, 2095 (2013); https://doi.org/10.1099/ijs.0.030965-0
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