1887

Abstract

A Gram-stain-negative, rod-shaped bacterium (strain K-39) was isolated from the thermophilic phase of the composting process for oyster mushroom substrate preparation. The strain grew at 40–80 °C (optimum, 65–75 °C), at pH 5–9 (optimum, pH 7), in media containing up to 1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K-39 formed a distinct lineage within the genus . Its closest cultivated relative was PRI 3838 (96.8 % similarity). The DNA G+C content of strain K-39 was 71.3 mol%. The new strain could be differentiated from the related taxa by not being able to hydrolyse starch. The predominant fatty acids of strain K-39 were iso-C and anteiso-C. Strain K-39 contained a lower amount of the fatty acid iso-C as compared to related species of the genus . The predominant respiratory quinone of the new isolate was menaquinone MK-8. On the basis of a taxonomic study using a polyphasic approach, strain K-39 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K-39 ( = DSM 21686 = NCAIM B 02340).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030866-0
2012-07-01
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1486.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030866-0&mimeType=html&fmt=ahah

References

  1. Bjornsdottir S. H. , Petursdottir S. K. , Hreggvidsson G. O. , Skirnisdottir S. , Hjorleifsdottir S. , Arnfinnsson J. , Kristjánsson J. K. . ( 2009; ). Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area. . Int J Syst Evol Microbiol 59:, 2962–2966. [CrossRef] [PubMed]
    [Google Scholar]
  2. Brock T. D. , Freeze H. . ( 1969; ). Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. . J Bacteriol 98:, 289–297.[PubMed]
    [Google Scholar]
  3. Casamayor E. O. , Schäfer H. , Bañeras L. , Pedrós-Alió C. , Muyzer G. . ( 2000; ). Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis. . Appl Environ Microbiol 66:, 499–508. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chung A. P. , Rainey F. A. , Valente M. , Nobre M. F. , da Costa M. S. . ( 2000; ). Thermus igniterrae sp. nov. and Thermus antranikianii sp. nov., two new species from Iceland. . Int J Syst Evol Microbiol 50:, 209–217. [CrossRef] [PubMed]
    [Google Scholar]
  7. Claus M. . ( 1992; ). A standardised Gram staining procedure. . World J Microbiol Biotechnol 8:, 451–452. [CrossRef]
    [Google Scholar]
  8. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  9. Cowan S. T. , Steel K. J. . ( 1974; ). Manual for the Identification of Medical Bacteria, , 2nd edn.. Cambridge:: Cambridge University Press;.
    [Google Scholar]
  10. da Costa M. S. , Rainey F. A. , Nobre M. F. . ( 2006; ). The genus Thermus and relatives. . In The Prokaryotes. A Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 797–812. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer;.
    [Google Scholar]
  11. Ferris M. J. , Muyzer G. , Ward D. M. . ( 1996; ). Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. . Appl Environ Microbiol 62:, 340–346.[PubMed]
    [Google Scholar]
  12. Groth I. , Schumann P. , Rainey F. A. , Martin K. , Schuetze B. , Augsten K. . ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hudson J. A. , Morgan H. W. , Daniel R. M. . ( 1987; ). Thermus filiformis sp. nov., a filamentous caldoactive bacterium. . Int J Syst Bacteriol 37:, 431–436. [CrossRef]
    [Google Scholar]
  14. Kristjánsson J. K. , Hjörleifsdóttir S. , Marteinsson V. T. , Alfredsson G. A. . ( 1994; ). Thermus scotoductus, sp. nov., a pigment-producing thermophilic bacterium from hot tap water in Iceland and including Thermus sp. X-1. . Syst Appl Microbiol 17:, 44–50. [CrossRef]
    [Google Scholar]
  15. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–147. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  18. Muyzer G. , de Waal E. C. , Uitterlinden A. G. . ( 1993; ). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. . Appl Environ Microbiol 59:, 695–700.[PubMed]
    [Google Scholar]
  19. Oshima T. , Imahori K. . ( 1974; ). Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. . Int J Syst Bacteriol 24:, 102–112. [CrossRef]
    [Google Scholar]
  20. Polz M. F. , Cavanaugh C. M. . ( 1998; ). Bias in template-to-product ratios in multitemplate PCR. . Appl Environ Microbiol 64:, 3724–3730.[PubMed]
    [Google Scholar]
  21. Pruesse E. , Quast C. , Knittel K. , Fuchs B. M. , Ludwig W. , Peplies J. , Glöckner F. O. . ( 2007; ). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB . . Nucleic Acids Res 35:, 7188–7196. [CrossRef] [PubMed]
    [Google Scholar]
  22. Quintela J. C. , Pittenauer E. , Allmaier G. , Arán V. , de Pedro M. A. . ( 1995; ). Structure of peptidoglycan from Thermus thermophilus HB8. . J Bacteriol 177:, 4947–4962.[PubMed]
    [Google Scholar]
  23. Rusznyák A. , Tóth E. M. , Schumann P. , Spröer C. , Makk J. , Szabó G. , Vladár P. , Márialigeti K. , Borsodi A. K. . ( 2011; ). Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond. . Int J Syst Evol Microbiol 61:, 1662–1666. [CrossRef] [PubMed]
    [Google Scholar]
  24. Santos M. A. , Williams R. A. D. , da Costa M. S. . ( 1989; ). Numerical taxonomy of Thermus isolates from hot springs in Portugal. . Syst Appl Microbiol 12:, 310–315. [CrossRef]
    [Google Scholar]
  25. Sipos R. , Székely A. J. , Palatinszky M. , Révész S. , Márialigeti K. , Nikolausz M. . ( 2007; ). Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis. . FEMS Microbiol Ecol 60:, 341–350. [CrossRef] [PubMed]
    [Google Scholar]
  26. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterisation. . In Methods for General and Molecular Bacteriology, pp. 603–711. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Stead D. E. , Sellwood J. E. , Wilson J. , Viney I. . ( 1992; ). Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. . J Appl Bacteriol 72:, 315–321. [CrossRef]
    [Google Scholar]
  28. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  29. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tarrand J. J. , Gröschel D. H. M. . ( 1982; ). Rapid, modified oxidase test for oxidase-variable bacterial isolates. . J Clin Microbiol 16:, 772–774.[PubMed]
    [Google Scholar]
  31. Vajna B. , Nagy A. , Sajben E. , Manczinger L. , Szijártó N. , Kádár Zs. , Bordás D. , Márialigeti K. . ( 2010; ). Microbial community structure changes during oyster mushroom substrate preparation. . Appl Microbiol Biotechnol 86:, 367–375. [CrossRef] [PubMed]
    [Google Scholar]
  32. Williams R. A. D. , Smith K. E. , Welch S. G. , Micallef J. , Sharp R. J. . ( 1995; ). DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori). . Int J Syst Bacteriol 45:, 495–499. [CrossRef] [PubMed]
    [Google Scholar]
  33. Williams R. A. D. , Smith K. E. , Welch S. G. , Micallef J. . ( 1996; ). Thermus oshimai sp. nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of a limited geographical distribution of Thermus species. . Int J Syst Bacteriol 46:, 403–408. [CrossRef] [PubMed]
    [Google Scholar]
  34. Yamada K. , Komagata K. . ( 1972; ). Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical and physiological characteristics. . J Gen Appl Microbiol 18:, 399–416. [CrossRef]
    [Google Scholar]
  35. Zhang X.-Q. , Ying Y. , Ye Y. , Xu X.-W. , Zhu X.-F. , Wu M. . ( 2010; ). Thermus arciformis sp. nov., a thermophilic species from a geothermal area. . Int J Syst Evol Microbiol 60:, 834–839. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030866-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030866-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error