1887

Abstract

A Gram-stain-negative, rod-shaped bacterium (strain K-39) was isolated from the thermophilic phase of the composting process for oyster mushroom substrate preparation. The strain grew at 40–80 °C (optimum, 65–75 °C), at pH 5–9 (optimum, pH 7), in media containing up to 1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K-39 formed a distinct lineage within the genus . Its closest cultivated relative was PRI 3838 (96.8 % similarity). The DNA G+C content of strain K-39 was 71.3 mol%. The new strain could be differentiated from the related taxa by not being able to hydrolyse starch. The predominant fatty acids of strain K-39 were iso-C and anteiso-C. Strain K-39 contained a lower amount of the fatty acid iso-C as compared to related species of the genus . The predominant respiratory quinone of the new isolate was menaquinone MK-8. On the basis of a taxonomic study using a polyphasic approach, strain K-39 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K-39 ( = DSM 21686 = NCAIM B 02340).

Funding
This study was supported by the:
  • Ministry of Agriculture and Rural Development (Hungary) (Award OMFB-00977/2005)
  • Ministry of Education and Culture, Hungary (Award DFÖ 0054/2009)
  • The European Union and the European Social Fund (Award TÁMOP 4.2.1./B-09/1/KMR-2010-0003)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030866-0
2012-07-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1486.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030866-0&mimeType=html&fmt=ahah

References

  1. Bjornsdottir S. H., Petursdottir S. K., Hreggvidsson G. O., Skirnisdottir S., Hjorleifsdottir S., Arnfinnsson J., Kristjánsson J. K. 2009; Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area. Int J Syst Evol Microbiol 59:2962–2966 [View Article][PubMed]
    [Google Scholar]
  2. Brock T. D., Freeze H. 1969; Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol 98:289–297[PubMed]
    [Google Scholar]
  3. Casamayor E. O., Schäfer H., Bañeras L., Pedrós-Alió C., Muyzer G. 2000; Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis. Appl Environ Microbiol 66:499–508 [View Article][PubMed]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  6. Chung A. P., Rainey F. A., Valente M., Nobre M. F., da Costa M. S. 2000; Thermus igniterrae sp. nov. and Thermus antranikianii sp. nov., two new species from Iceland. Int J Syst Evol Microbiol 50:209–217 [View Article][PubMed]
    [Google Scholar]
  7. Claus M. 1992; A standardised Gram staining procedure. World J Microbiol Biotechnol 8:451–452 [View Article]
    [Google Scholar]
  8. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  10. da Costa M. S., Rainey F. A., Nobre M. F. 2006; The genus Thermus and relatives. In The Prokaryotes. A Handbook on the Biology of Bacteria, 3rd edn. vol. 7 pp. 797–812 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  11. Ferris M. J., Muyzer G., Ward D. M. 1996; Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl Environ Microbiol 62:340–346[PubMed]
    [Google Scholar]
  12. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
    [Google Scholar]
  13. Hudson J. A., Morgan H. W., Daniel R. M. 1987; Thermus filiformis sp. nov., a filamentous caldoactive bacterium. Int J Syst Bacteriol 37:431–436 [View Article]
    [Google Scholar]
  14. Kristjánsson J. K., Hjörleifsdóttir S., Marteinsson V. T., Alfredsson G. A. 1994; Thermus scotoductus, sp. nov., a pigment-producing thermophilic bacterium from hot tap water in Iceland and including Thermus sp. X-1. Syst Appl Microbiol 17:44–50 [View Article]
    [Google Scholar]
  15. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–147 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [View Article]
    [Google Scholar]
  18. Muyzer G., de Waal E. C., Uitterlinden A. G. 1993; Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700[PubMed]
    [Google Scholar]
  19. Oshima T., Imahori K. 1974; Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int J Syst Bacteriol 24:102–112 [View Article]
    [Google Scholar]
  20. Polz M. F., Cavanaugh C. M. 1998; Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol 64:3724–3730[PubMed]
    [Google Scholar]
  21. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB . Nucleic Acids Res 35:7188–7196 [View Article][PubMed]
    [Google Scholar]
  22. Quintela J. C., Pittenauer E., Allmaier G., Arán V., de Pedro M. A. 1995; Structure of peptidoglycan from Thermus thermophilus HB8. J Bacteriol 177:4947–4962[PubMed]
    [Google Scholar]
  23. Rusznyák A., Tóth E. M., Schumann P., Spröer C., Makk J., Szabó G., Vladár P., Márialigeti K., Borsodi A. K. 2011; Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond. Int J Syst Evol Microbiol 61:1662–1666 [View Article][PubMed]
    [Google Scholar]
  24. Santos M. A., Williams R. A. D., da Costa M. S. 1989; Numerical taxonomy of Thermus isolates from hot springs in Portugal. Syst Appl Microbiol 12:310–315 [View Article]
    [Google Scholar]
  25. Sipos R., Székely A. J., Palatinszky M., Révész S., Márialigeti K., Nikolausz M. 2007; Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis. FEMS Microbiol Ecol 60:341–350 [View Article][PubMed]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R. 1994; Phenotypic characterisation. In Methods for General and Molecular Bacteriology pp. 603–711 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Stead D. E., Sellwood J. E., Wilson J., Viney I. 1992; Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72:315–321 [View Article]
    [Google Scholar]
  28. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  30. Tarrand J. J., Gröschel D. H. M. 1982; Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 16:772–774[PubMed]
    [Google Scholar]
  31. Vajna B., Nagy A., Sajben E., Manczinger L., Szijártó N., Kádár Zs., Bordás D., Márialigeti K. 2010; Microbial community structure changes during oyster mushroom substrate preparation. Appl Microbiol Biotechnol 86:367–375 [View Article][PubMed]
    [Google Scholar]
  32. Williams R. A. D., Smith K. E., Welch S. G., Micallef J., Sharp R. J. 1995; DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori). Int J Syst Bacteriol 45:495–499 [View Article][PubMed]
    [Google Scholar]
  33. Williams R. A. D., Smith K. E., Welch S. G., Micallef J. 1996; Thermus oshimai sp. nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of a limited geographical distribution of Thermus species. Int J Syst Bacteriol 46:403–408 [View Article][PubMed]
    [Google Scholar]
  34. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical and physiological characteristics. J Gen Appl Microbiol 18:399–416 [View Article]
    [Google Scholar]
  35. Zhang X.-Q., Ying Y., Ye Y., Xu X.-W., Zhu X.-F., Wu M. 2010; Thermus arciformis sp. nov., a thermophilic species from a geothermal area. Int J Syst Evol Microbiol 60:834–839 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030866-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030866-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error