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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_3

sp. nov., isolated from a freshwater pipe Free

Abstract

A Gram-staining-negative, aerobic to microaerophilic, rod-shaped, red-coloured bacterium, strain T4, was isolated from a freshwater pipe on Tenerife island. A polyphasic taxonomic study was performed in order to characterize the strain in detail. The isolate is surrounded by a slime capsule, occurs singly, in the form of short chains, or in aggregates, and exhibits catalase and oxidase activities. Growth was observed at 15–42 °C. Optimum growth occurred at pH 8 with mono- and disaccharides, followed by polysaccharides and deoxysaccharides, but the bacterium utilized only a restricted spectrum of alcohols, alditols, amides, amines, carboxylic acids and amino acids. Strain T4 tolerated concentrations of 0–4 % (w/v) NaCl and contained MK-7 as predominant isoprenoid quinone as well as carotenoids, but lacked pigments of the flexirubin type. The predominant fatty acids were iso-C (32.2 %), summed feature 3 (Cω6 and/or Cω7; 22.5 %), and iso-C 3-OH (7.9 %). Major polar lipids were phosphatidylethanolamine, phospholipids, aminophospholipids and other lipids of unknown character. The DNA GC content was approximately 41.8 mol%. The sequence of the 16S-rRNA gene assigned strain T4 to the CFB group, forming a coherent cluster with species of the genus with the highest similarity of 98.8 % to A8-7. DNA–DNA hybridization revealed 37.5 % relatedness to strain A8-7. Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain T4 should be placed into the genus as a novel species, for which the name sp. nov. (type strain T4  = DSM 19759  = LMG 24398  = NCIMB 14399) is proposed.

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2012-03-01
2024-04-24
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Algoriphagus aquaeductus sp. nov., isolated from a freshwater pipe
Int J Syst Evol Microbiol 62, 675 (2012); https://doi.org/10.1099/ijs.0.030809-0
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