1887

Abstract

A Gram-staining-negative, non-motile, non-spore-forming bacterial strain, DPG-3, was isolated from seawater from the South Sea in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain DPG-3 grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain DPG-3 fell within a clade comprising species and appeared most closely related to JC 2051 (96.1 %16S rRNA gene sequence similarity) and S1-3 (96.4 %). The type strains of other species showed 16S rRNA gene sequence similarities of 92.9–96.0 % with strain DPG-3. The predominant menaquinone of strain DPG-3 was MK-7. The major fatty acids were iso-C and iso-C 2-OH and/or Cω7 (summed feature 3). The major polar lipids detected in strain DPG-3 were phosphatidylcholine, phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content was 44.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPG-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DPG-3 ( = KCTC 23419 = CCUG 60523).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030791-0
2012-03-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/3/575.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030791-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  2. Bowman J. P. , Nichols C. M. , Gibson J. A. E. . ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. . Int J Syst Evol Microbiol 53:, 1343–1355. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef] [PubMed]
    [Google Scholar]
  4. Copa-Patiño J. L. , Arenas M. , Soliveri J. , Sánchez-Porro C. , Ventosa A. . ( 2008; ). Algoriphagus hitonicola sp. nov., isolated from an athalassohaline lagoon. . Int J Syst Evol Microbiol 58:, 424–428. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  6. Euzéby J. P. . ( 1997; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47:, 590–592. [CrossRef] [PubMed]
    [Google Scholar]
  7. Euzéby J. P. . ( 2010; ). List of prokaryotic names with standing in nomenclature. . http://www.cict.fr
  8. Komagata K. , Suzuki K.-I. . ( 1987; ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  9. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  10. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  11. Liu Y. , Li H. , Jiang J. T. , Liu Y. H. , Song X. F. , Xu C. J. , Liu Z. P. . ( 2009; ). Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. . Int J Syst Evol Microbiol 59:, 1759–1763. [CrossRef] [PubMed]
    [Google Scholar]
  12. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  13. Nedashkovskaya O. I. , Vancanneyt M. , Van Trappen S. , Vandemeulebroecke K. , Lysenko A. M. , Rohde M. , Falsen E. , Frolova G. M. , Mikhailov V. V. , Swings J. . ( 2004; ). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. . Int J Syst Evol Microbiol 54:, 1757–1764. [CrossRef] [PubMed]
    [Google Scholar]
  14. Nedashkovskaya O. I. , Kim S. B. , Kwon K. K. , Shin D. S. , Luo X. , Kim S.-J. , Mikhailov V. V. . ( 2007; ). Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. . Int J Syst Evol Microbiol 57:, 1988–1994. [CrossRef] [PubMed]
    [Google Scholar]
  15. Park S. , Kang S.-J. , Oh K.-H. , Oh T.-K. , Yoon J.-H. . ( 2010; ). Algoriphagus lutimaris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 60:, 200–204. [CrossRef] [PubMed]
    [Google Scholar]
  16. Reichenbach H. . ( 1992; ). The order Cytophagales. . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, , 2nd edn., pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.
    [Google Scholar]
  17. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  18. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  19. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  20. Yi H. , Chun J. . ( 2004; ). Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54:, 157–162.[PubMed] [CrossRef]
    [Google Scholar]
  21. Yoon J.-H. , Kim H. , Kim S.-B. , Kim H.-J. , Kim W. Y. , Lee S. T. , Goodfellow M. , Park Y.-H. . ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  22. Yoon J.-H. , Lee S. T. , Park Y.-H. . ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef] [PubMed]
    [Google Scholar]
  23. Yoon J.-H. , Kim I.-G. , Shin D.-Y. , Kang K. H. , Park Y.-H. . ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53:, 53–57. [CrossRef] [PubMed]
    [Google Scholar]
  24. Yoon J.-H. , Kang S.-J. , Jung S.-Y. , Lee C.-H. , Oh T.-K. . ( 2005a; ). Algoriphagus yeomjeoni sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea. . Int J Syst Evol Microbiol 55:, 865–870. [CrossRef] [PubMed]
    [Google Scholar]
  25. Yoon J.-H. , Kang S.-J. , Oh T.-K. . ( 2005b; ). Algoriphagus locisalis sp. nov., isolated from a marine solar saltern. . Int J Syst Evol Microbiol 55:, 1635–1639. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yoon J.-H. , Lee M.-H. , Kang S.-J. , Oh T.-K. . ( 2006; ). Algoriphagus terrigena sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56:, 777–780. [CrossRef] [PubMed]
    [Google Scholar]
  27. Young C.-C. , Lin S.-Y. , Arun A. B. , Shen F.-T. , Chen W.-M. , Rekha P. D. , Langer S. , Busse H.-J. , Wu Y.-H. , Kämpfer P. . ( 2009; ). Algoriphagus olei sp. nov., isolated from oil-contaminated soil. . Int J Syst Evol Microbiol 59:, 2909–2915. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030791-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030791-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error