1887

Abstract

A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4, was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20–40 °C (optimum, 30–37 °C), at pH 7.0–10.0 (optimum, pH 7.0–9.0) and with 0–3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4, together with CS-6 (96.4 % sequence similarity), B8 (96.1 %), B4 (95.5 %) and B6 (94.7 %), formed a deep line within the order . Strain ABP-4 contained summed feature 3 (comprising C ω7 and/or C ω6), C ω7 and C as predominant fatty acids. The major cellular hydroxy fatty acid was C 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA–DNA relatedness of strain ABP-4 with respect to recognized species of the genus was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ABP-4 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is ABP-4 ( = BCRC 80167 = KCTC 23306).

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2012-03-01
2019-12-15
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References

  1. Anzai Y. , Kudo Y. , Oyaizu H. . ( 1997; ). The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. . Int J Syst Bacteriol 47:, 249–251. [CrossRef] [PubMed]
    [Google Scholar]
  2. Breznak J. A. , Costilow R. N. . ( 1994; ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Chen W. M. , Laevens S. , Lee T. M. , Coenye T. , De Vos P. , Mergeay M. , Vandamme P. . ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51:, 1729–1735. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  6. Embley T. M. , Wait R. . ( 1994; ). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M. , O’Donnell A. G. . . England:: John Wiley & Sons Ltd;.
    [Google Scholar]
  7. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. . Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Gomila M. , Bowien B. , Falsen E. , Moore E. R. B. , Lalucat J. . ( 2008; ). Description of Roseateles aquatilis sp. nov. and Roseateles terrae sp. nov., in the class Betaproteobacteria, and emended description of the genus Roseateles . . Int J Syst Evol Microbiol 58:, 6–11. [CrossRef] [PubMed]
    [Google Scholar]
  11. Gomila M. , Pinhassi J. , Falsen E. , Moore E. R. B. , Lalucat J. . ( 2010; ). Kinneretia asaccharophila gen. nov., sp. nov., isolated from a freshwater lake, a member of the Rubrivivax branch of the family Comamonadaceae . . Int J Syst Evol Microbiol 60:, 809–814. [CrossRef] [PubMed]
    [Google Scholar]
  12. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  13. Kalmbach S. , Manz W. , Wecke J. , Szewzyk U. . ( 1999; ). Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system. . Int J Syst Bacteriol 49:, 769–777. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  15. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  16. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lechner U. , Brodkorb D. , Geyer R. , Hause G. , Härtig C. , Auling G. , Fayolle-Guichard F. , Piveteau P. , Müller R. H. , Rohwerder T. . ( 2007; ). Aquincola tertiaricarbonis gen. nov., sp. nov., a tertiary butyl moiety-degrading bacterium. . Int J Syst Evol Microbiol 57:, 1295–1303. [CrossRef] [PubMed]
    [Google Scholar]
  18. Lin M. C. , Jiang S. R. , Chou J. H. , Arun A. B. , Young C. C. , Chen W. M. . ( 2009; ). Aquabacterium fontiphilum sp. nov., isolated from spring water. . Int J Syst Evol Microbiol 59:, 681–685. [CrossRef] [PubMed]
    [Google Scholar]
  19. Maidak B. L. , Cole J. R. , Lilburn T. G. , Parker C. T. Jr , Saxman P. R. , Farris R. J. , Garrity G. M. , Olsen G. J. , Schmidt T. M. , Tiedje J. M. . ( 2001; ). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29:, 173–174. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the GC content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  21. Nokhal T. H. , Schlegel H. G. . ( 1983; ). Taxonomic study of Paracoccus denitrijicans . . Int J Syst Bacteriol 33:, 26–37. [CrossRef]
    [Google Scholar]
  22. Powers E. M. . ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  23. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  24. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Suyama T. , Shigematsu T. , Takaichi S. , Nodasaka Y. , Fujikawa S. , Hosoya H. , Tokiwa Y. , Kanagawa T. , Hanada S. . ( 1999; ). Roseateles depolymerans gen. nov., sp. nov., a new bacteriochlorophyll a-containing obligate aerobe belonging to the β-subclass of the Proteobacteria . . Int J Syst Bacteriol 49:, 449–457. [CrossRef] [PubMed]
    [Google Scholar]
  26. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  27. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches of bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
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