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Volume 62, Issue Pt_3

sp. nov., isolated from a freshwater spring Free

Abstract

A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4, was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20–40 °C (optimum, 30–37 °C), at pH 7.0–10.0 (optimum, pH 7.0–9.0) and with 0–3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4, together with CS-6 (96.4 % sequence similarity), B8 (96.1 %), B4 (95.5 %) and B6 (94.7 %), formed a deep line within the order . Strain ABP-4 contained summed feature 3 (comprising C ω7 and/or C ω6), C ω7 and C as predominant fatty acids. The major cellular hydroxy fatty acid was C 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA–DNA relatedness of strain ABP-4 with respect to recognized species of the genus was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ABP-4 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is ABP-4 ( = BCRC 80167 = KCTC 23306).

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2012-03-01
2024-04-24
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Aquabacterium limnoticum sp. nov., isolated from a freshwater spring
Int J Syst Evol Microbiol 62, 698 (2012); https://doi.org/10.1099/ijs.0.030635-0
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