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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_5

sp. nov., a diazotroph from agricultural soil Free

Abstract

A Gram-negative, spiral or rod-shaped, non-spore-forming diazotrophic bacterium, designated CC-Nfb-7, was isolated from agricultural soil in Yunlin County, Taiwan. 16S rRNA gene sequence analysis showed that strain CC-Nfb-7 was most closely related to DSM 1690 (97.4 % 16S rRNA gene sequence similarity), IMMIB AFH-6 (96.8 %) and JCM 21588 (96.6 %); <96.5 % 16S rRNA gene sequence similarity was found with all other members of the genus . DNA–DNA relatedness between strain CC-Nfb-7 and DSM 1690, DSM 19657 and JCM 21588 was 38.9, 30.1 and 31.8 %, respectively. The respiratory quinone was ubiquinone Q-10. The major fatty acids were summed feature 8 (consisting of Cω7 and/or Cω6), summed feature 3 (consisting of Cω7 and/or Cω6), summed feature 2 (consisting of C 3-OH and/or iso-C I), C, C 2-OH and C 3-OH. The polar lipids consisted mainly of phosphatidylglycerol, phosphatidylcholine and one unidentified phospholipid. Furthermore, moderate amounts of phosphatidylethanolamine, phosphatidyldimethylethanolamine and one unidentified aminophospholipid were also detected. Strain CC-Nfb-7 could be distinguished from members of phylogenetically related species by differences in phenotypic properties. On the basis of morphological, chemotaxonomic and phylogenetic data, strain CC-Nfb-7 represents a novel species within the genus , for which we propose the name sp. nov. The type strain is CC-Nfb-7 ( = BCRC 80273 = JCM 17639 = DSM 24137).

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Funding
This study was supported by the:
  • National Science Council
  • Council of Agriculture, Executive Yuan
  • Ministry of Education, Taiwan, ROC
© 2012 IUMS
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2012-05-01
2025-06-19
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Azospirillum formosense sp. nov., a diazotroph from agricultural soil
Int J Syst Evol Microbiol 62, 1185 (2012); https://doi.org/10.1099/ijs.0.030585-0
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