1887

Abstract

Seven melanogenic -like strains, KMM 3562, KMM 3536, KMM 3537, KMM 3538, KMM 3539, KMM 3615 and KMM 3629, which expressed tyrosinases were isolated from sea-water samples collected from different locations in Amursky Bay (Sea of Japan, Pacific Ocean) and characterized to clarify their taxonomic position. By 16S rRNA gene sequence analysis, the bacteria were shown to belong to the genus . The G+C content of the DNAs of the strains was 41–43 mol%. The level of DNA similarity among these strains was conspecific (92–97 %), indicating that they represented a single genospecies. However, DNA from the strains isolated from sea water showed only 63–65 % genetic relatedness with the DNA of the type strain . The novel organisms grew mainly between 4 and 30 °C, were neutrophilic and slightly halophilic (four strains had a narrow range of growth between 3 and 6 % NaCl, w/v), were haemolytic and cytotoxic and were able to degrade starch, gelatin and Tween 80. The predominant fatty acids, including 16 : 0, 16 : 17, 17 : 17 and 18 : 17, were typical of the genus . The phylogenetic, genetic and physiological properties of the seven strains placed them within a novel species, sp. nov., the type strain of which is SW19 (=KMM 3562=LMG 22059).

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2004-09-01
2021-04-10
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References

  1. Anzai Y., Kim H., Park J. Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  2. Azegami K., Nishiyama K., Watanabe Y., Kadota I., Ohuchi A., Fukazawa C. 1987; Pseudomonas plantarii sp. nov., the causal agent of rice seeding blight. Int J Syst Bacteriol 37:144–152 [CrossRef]
    [Google Scholar]
  3. Barry A. I. 1980; Procedures and theoretical considerations for testing antimicrobial agents in agar media. In Antibiotics in Laboratory Medicine pp  10–16 Edited by Logan V. Baltimore: William & Wilkins;
    [Google Scholar]
  4. Bowman J. P., Cavanagh J., Austin J. J., Sanderson K. 1996; Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46:841–848 [CrossRef]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Gascuel O. 1997; BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14:685–695 [CrossRef]
    [Google Scholar]
  7. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas , Shewanella , and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and twelve new species combinations. Int J Syst Bacteriol 45:755–761 [CrossRef]
    [Google Scholar]
  8. Ivanova E. P., Mikhailov V. V. 2001; A new family of Alteromonadaceae fam. nov., including the marine proteobacteriaAlteromonas , Pseudoalteromonas , Idiomarina , and Colwellia . Microbiology (English translation of Mikrobiologiya ) 7015–23
    [Google Scholar]
  9. Ivanova E. P., Kiprianova E. A., Mikhailov V. V., Levanova F. G., Garagulya A. G., Gorshkova N. M., Yumoto N., Yoshikawa S. 1996; Characterization and identification of marine Alteromonas nigrifaciens strains and emendation of the description. Int J Syst Bacteriol 46:223–228 [CrossRef]
    [Google Scholar]
  10. Ivanova E. P., Kiprianova E. A., Mikhailov V. V. 7 other authors 1998; Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48:247–256 [CrossRef]
    [Google Scholar]
  11. Ivanova E. P., Zhukova N. V., Svetashev V. I., Gorshkova N. M., Kurilenko V. V., Frolova G. M., Mikhailov V. V. 2000a; Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas -like proteobacteria. Curr Microbiol 41:341–345 [CrossRef]
    [Google Scholar]
  12. Ivanova E. P., Chun J., Romanenko L. A., Matte M. E., Mikhailov V. V., Frolova G. M., Huq A., Colwell R. R. 2000b; Reclassification of Alteromonas distincta Romanenko et al . 1995 as Pseudoalteromonas distincta comb. nov. Int J Syst Evol Microbiol 50:141–144 [CrossRef]
    [Google Scholar]
  13. Ivanova E. P., Sawabe T., Lysenko A. M., Gorshkova N. M., Hayashi K., Zhukova N. V. I., Nicolau D. V., Christen R., Mikhailov V. V. 2002; Pseudoalteromonas translucida sp. nov. and Pseudoalteromonas paragorgicola sp. nov., and emended description of the genus. Int J Syst Evol Microbiol 52:1759–1766 [CrossRef]
    [Google Scholar]
  14. Ivanova E. P., Bakunina I. Y., Nedashkovskaya O. I., Gorshkova N. M., Alexeeva Y. V., Zelepuga E. A., Zvaygintseva T. N., Nicolau D. V., Mikhailov D. V. 2003; Ecophysiological variabilities in ectohydrolytic enzyme activities of some Pseudoalteromonas species, P. citrea , P.issachenkonii , and P. nigrifaciens . Curr Microbiol 46:6–10 [CrossRef]
    [Google Scholar]
  15. Ivanova E. P., Gorshkova N. M., Sawabe T. 8 other authors 2004; Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish ( Stellaster equestris ) and sea grass ( Zostera marina ). Int J Syst Evol Microbiol 54:475–480 [CrossRef]
    [Google Scholar]
  16. Lucas-Elío P., Solano F., Sanchez-Amat A. 2002; Regulation of polyphenol oxidase activities and melanin synthesis in Marinomonas mediterranea : identification of ppoS , a gene encoding a sensor histidine kinase. Microbiology 148:2457–2466
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  18. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  19. Perrière G., Gouy M. 1996; WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369 [CrossRef]
    [Google Scholar]
  20. Pomerantz S. H., Murphy V. V. 1974; Purification and properties of tyrosinases from Vibrio tyrosinaticus . Arch Biochem Biophys 160:73–82 [CrossRef]
    [Google Scholar]
  21. Prokof'eva N. G., Chaikina E. L., Kicha A. A., Ivanchina N. V. 2003; Biological activities of steroid glycosides from starfish. Comp Biochem Physiol 134:695–701 [CrossRef]
    [Google Scholar]
  22. Romanenko L. A., Mikhailov V. V., Lysenko A. M., Stepanenko V. I. 1995; A new species of melanin-producing bacteria of the genus Alteromonas . Microbiology (English translation of Mikrobiologiya) 6474–77
    [Google Scholar]
  23. Sasaki T., Uchida N. A., Uchida H., Takasuka N., Kamiya H., Endo Y., Tanaka M., Hayashi T., Shimizu Y. 1985; Antitumor activity of aqueous extracts of marine animals. J Pharmacobiodyn 8:969–974 [CrossRef]
    [Google Scholar]
  24. Sasser M. 1997 MIDI: Technical note 102 Newark, DE: Microbial ID;
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt F. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Svetashev V. I., Vysotskii M. V., Ivanova E. P., Mikhailov V. V. 1995; Cellular fatty acid of Alteromonas species. Syst Appl Microbiol 18:37–43 [CrossRef]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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