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Abstract

A Gram-positive, aerobic actinobacterium with high chromate [Cr(VI)]-reducing ability, designated strain Q5-1, was isolated from manganese mining soil in Hunan Province, central-south China. The organism formed branching hyphae and contained -diaminopimelic acid in the cell-wall peptidoglycan. The polar lipid profile was characterized by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The major fatty acids were iso-C, iso-C, anteiso-C and iso-C, and the predominant menaquinone was MK-8(H). The genomic DNA G+C content was 71.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Q5-1 was closely related to DSM 43043 ( = NRRL B-3866) and KV-657 ( = NRRL B-24470) with similarities of 96.6 and 96.4 %, respectively. Comparison of phenotypic, biochemical and chemotaxonomic characters of strain Q5-1 and phylogenetically related strains revealed that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Q5-1 ( = KCTC 19811 = CCTCC AA 2010019 = CGMCC 1.10750 = NRRL B-59521). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31010103903)
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2012-02-01
2020-12-04
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