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Abstract

A strictly aerobic, non-motile, rod-shaped actinomycete strain, named JC2055, was isolated from a sediment sample of getbol, the tidal flat of Korea. 16S rDNA analysis revealed that the getbol isolate belonged to the genus with the highest sequence similarity to GW-9 (95·5 %). The major menaquinone was MK-8(H) and the predominant cellular fatty acids were iso-16 : 0 and 17 : 18. Tuberculostearic acid was absent. The G+C content of DNA was 72 mol%. Based on the morphological, physiological, biochemical and chemotaxonomical data presented in this study, strain JC2055 can be readily differentiated from other validly named species. The name sp. nov. is proposed for the isolate. The type strain is JC2055 (=IMSNU 14028=KCTC 9920=JCM 12124).

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2004-07-01
2019-10-13
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References

  1. Baumann, L., Baumann, P., Mandel, M. & Allen, R. D. ( 1972; ). Taxonomy of aerobic marine eubacteria. J Bacteriol 110, 402–429.
    [Google Scholar]
  2. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  3. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  4. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y. ( 1957; ). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49, 25–68.[CrossRef]
    [Google Scholar]
  5. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  6. Collins, M. D., Cockcroft, S. & Wallbanks, S. ( 1994; ). Phylogenetic analysis of a new ll-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44, 523–526.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5.1. Department of Genetics, University of Washington, Seattle, USA.
  9. Fitch, W. M. ( 1972; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.
    [Google Scholar]
  10. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  11. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  12. Gosink, J. J., Woese, C. R. & Staley, J. T. ( 1998; ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef]
    [Google Scholar]
  13. Hendrickson, D. A. & Krenz, M. M. ( 1991; ). Reagents and stains. In Manual of Clinical Microbiology, pp. 1289–1314. Edited by A. Balows, W. J. Hausler, Jr, K. L. Herrmann, H. D. Isenberg & H. J. Shadomy. Washington, DC: American Society for Microbiology.
  14. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M. ( 2000; ). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[CrossRef]
    [Google Scholar]
  16. Lyman, J. & Fleming, R. H. ( 1940; ). Composition of sea water. J Mar Res 3, 134–146.
    [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  18. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athayle, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  19. O'Donnell, A. G., Goodfellow, M. & Minnikin, D. E. ( 1982; ). Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus Nocardioides (Prauser) emend. O'Donnell et al. as Nocardioides simplex comb. nov. Arch Microbiol 133, 323–329.[CrossRef]
    [Google Scholar]
  20. Prauser, H. ( 1976; ). Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef]
    [Google Scholar]
  21. Prauser, H. ( 1986; ). Genus Nocardioides Prauser 1976 , 61AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1481–1485. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  22. Rosselló-Mora, R. & Amann, R. ( 2001; ). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef]
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Washington, DC: American Society for Microbiology.
  25. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  26. Staley, J. T. ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942.
    [Google Scholar]
  27. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  28. Suzuki, K.-I. & Komagata, K. ( 1983; ). Pimelobacter gen. nov., a new genus of coryneform bacteria with ll-diaminopimelic acid in the cell wall. J Gen Appl Microbiol 29, 59–71.[CrossRef]
    [Google Scholar]
  29. Swofford, D. L. ( 1998; ). Phylogenetic analysis using parsimony (paup). Version 4. Sunderland, MA: Sinauer Associates.
  30. Urzi, C., Salamone, P., Schumann, P. & Stackebrandt, E. ( 2000; ). Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50, 529–536.[CrossRef]
    [Google Scholar]
  31. Yi, H. & Chun, J. ( 2002; ). Remarkable cultured bacterial biodiversity in getbol, the tidal flat of Korea. In Proceedings of the International Union of Microbiological Societies World Congresses, p. 165. Paris: IUMS.
  32. Yoon, J. H., Rhee, S. K., Lee, J. S., Park, Y. H. & Lee, S. T. ( 1997; ). Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47, 933–938.[CrossRef]
    [Google Scholar]
  33. Yoon, J. H., Cho, Y. G., Lee, S. T., Suzuki, K., Nakase, T. & Park, Y. H. ( 1999; ). Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49, 675–680.[CrossRef]
    [Google Scholar]
  34. Yoon, J.-H., Kim, I.-G., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54, 71–75.[CrossRef]
    [Google Scholar]
  35. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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vol. , part 4, pp. 1295 - 1299

Cellular fatty acid composition (%) of strain JC2055 and Nocardioides plantarum IMSNU 22067 [PDF](121 KB)



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