1887

Abstract

A novel actinomycete strain, designated CW 59, was isolated from a polluted forest soil sample in Anhui Province, China. Cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. The major fatty acids were ai-C (34.7 %), i-C (11.6 %) and ai-C (14.9 %); the predominant respiratory quinone was MK-9(H), with MK-8(H) present as a minor component. The polar lipid composition of strain CW 59 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and phosphatidylmonomethylethanolamine (minor component). The whole-cell sugars contained galactose, mannose, ribose and glucose; the major amino acids of the cell-wall were lysine, alanine and glutamic acid. The genomic DNA G+C content was 66.9 mol%. Phylogenetic analysis showed that CW 59 belonged to the genus and grouped with members of the species , , and . 16S rRNA gene sequence similarities of CW 59 to DSM 20127, CW 108, LC10 and LC13 were 99.5, 99.3, 98.2 and 98.0 %, respectively. DNA–DNA hybridization of the isolate showed relatedness values of 58.3 % (DSM 20127), 41.8 % (CW 108), 21.6 % (LC10) and 25.5 % (LC13) with its four closest neighbours. The taxonomic relationships of strains LC10 and LC13 with the genus were further clarified by means of a direct experimental comparison; results showed that strains LC10 and LC13 showed the same major fatty acid, polar lipid, cell-wall amino acid, whole-cell sugar and respiratory quinone compositions as members of the genus . Based on phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that: strain CW 59 represents a novel species of the genus , sp. nov., with CW 59 ( = CCTCC AB 207193 = KCTC 19389) as the type strain; and the type strains of and should be reclassified as comb. nov. and comb. nov., respectively. An emended description of the genus is given.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31000001)
  • China Postdoctoral Science Foundation (Award 20090461410)
  • Zhejiang Province General Program Grant (Award 2010C32024)
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2012-04-01
2024-04-25
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References

  1. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  2. Ding L., Hirose T., Yokota A. 2009; Four novel Arthrobacter species isolated from filtration substrate. Int J Syst Evol Microbiol 59:856–862 [View Article][PubMed]
    [Google Scholar]
  3. Felsenstein J. 1985; Conference limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [View Article]
    [Google Scholar]
  4. Hu H. Y., Lim B. R., Goto N., Fujie K. 2001; Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 47:17–24 [View Article][PubMed]
    [Google Scholar]
  5. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  6. Jahnke K. D. 1992; BASIC computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [View Article]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  9. Kuhn D. A., Starr M. P. 1960; Arthrobacter atrocyaneus, n. sp., and its blue pigment. Arch Mikrobiol 36:175–181 [View Article][PubMed]
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  11. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  15. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newslett 20:1–6
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  18. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  20. Wieser M., Denner E. B. M., Kämpfer P., Schumann P., Tindall B., Steiner U., Vybiral D., Lubitz W., Maszenan A. M. other authors 2002; Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Int J Syst Evol Microbiol 52:629–637[PubMed]
    [Google Scholar]
  21. Yoon J. H., Kim I. G., Kang K. H., Oh T. K., Park Y. H. 2003; Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53:1297–1303 [View Article][PubMed]
    [Google Scholar]
  22. Zhou Y., Dong J., Wang X., Huang X., Zhang K. Y., Zhang Y. Q., Guo Y. F., Lai R., Li W. J. 2007; Chryseobacterium flavum sp. nov., isolated from polluted soil. Int J Syst Evol Microbiol 57:1765–1769 [View Article][PubMed]
    [Google Scholar]
  23. Zhou Y., Wei W., Wang X., Lai R. 2009; Proposal of Sinomonas flava gen. nov., sp. nov., and description of Sinomonas atrocyanea comb. nov. to accommodate Arthrobacter atrocyaneus . Int J Syst Evol Microbiol 59:259–263 [View Article][PubMed]
    [Google Scholar]
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