1887

Abstract

A novel actinomycete strain, designated CW 59, was isolated from a polluted forest soil sample in Anhui Province, China. Cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. The major fatty acids were ai-C (34.7 %), i-C (11.6 %) and ai-C (14.9 %); the predominant respiratory quinone was MK-9(H), with MK-8(H) present as a minor component. The polar lipid composition of strain CW 59 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and phosphatidylmonomethylethanolamine (minor component). The whole-cell sugars contained galactose, mannose, ribose and glucose; the major amino acids of the cell-wall were lysine, alanine and glutamic acid. The genomic DNA G+C content was 66.9 mol%. Phylogenetic analysis showed that CW 59 belonged to the genus and grouped with members of the species , , and . 16S rRNA gene sequence similarities of CW 59 to DSM 20127, CW 108, LC10 and LC13 were 99.5, 99.3, 98.2 and 98.0 %, respectively. DNA–DNA hybridization of the isolate showed relatedness values of 58.3 % (DSM 20127), 41.8 % (CW 108), 21.6 % (LC10) and 25.5 % (LC13) with its four closest neighbours. The taxonomic relationships of strains LC10 and LC13 with the genus were further clarified by means of a direct experimental comparison; results showed that strains LC10 and LC13 showed the same major fatty acid, polar lipid, cell-wall amino acid, whole-cell sugar and respiratory quinone compositions as members of the genus . Based on phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that: strain CW 59 represents a novel species of the genus , sp. nov., with CW 59 ( = CCTCC AB 207193 = KCTC 19389) as the type strain; and the type strains of and should be reclassified as comb. nov. and comb. nov., respectively. An emended description of the genus is given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030361-0
2012-04-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/4/764.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030361-0&mimeType=html&fmt=ahah

References

  1. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ding L. , Hirose T. , Yokota A. . ( 2009; ). Four novel Arthrobacter species isolated from filtration substrate. . Int J Syst Evol Microbiol 59:, 856–862. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J. . ( 1985; ). Conference limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–789. [CrossRef]
    [Google Scholar]
  4. Hu H. Y. , Lim B. R. , Goto N. , Fujie K. . ( 2001; ). Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. . J Microbiol Methods 47:, 17–24. [CrossRef] [PubMed]
    [Google Scholar]
  5. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  6. Jahnke K. D. . ( 1992; ). BASIC computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  7. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  9. Kuhn D. A. , Starr M. P. . ( 1960; ). Arthrobacter atrocyaneus, n. sp., and its blue pigment. . Arch Mikrobiol 36:, 175–181. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  11. Li W. J. , Xu P. , Schumann P. , Zhang Y. Q. , Pukall R. , Xu L. H. , Stackebrandt E. , Jiang C. L. . ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  12. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  13. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  15. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newslett 20:, 1–6.
    [Google Scholar]
  16. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  18. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  19. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  20. Wieser M. , Denner E. B. M. , Kämpfer P. , Schumann P. , Tindall B. , Steiner U. , Vybiral D. , Lubitz W. , Maszenan A. M. . & other authors ( 2002; ). Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). . Int J Syst Evol Microbiol 52:, 629–637.[PubMed]
    [Google Scholar]
  21. Yoon J. H. , Kim I. G. , Kang K. H. , Oh T. K. , Park Y. H. . ( 2003; ). Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 53:, 1297–1303. [CrossRef] [PubMed]
    [Google Scholar]
  22. Zhou Y. , Dong J. , Wang X. , Huang X. , Zhang K. Y. , Zhang Y. Q. , Guo Y. F. , Lai R. , Li W. J. . ( 2007; ). Chryseobacterium flavum sp. nov., isolated from polluted soil. . Int J Syst Evol Microbiol 57:, 1765–1769. [CrossRef] [PubMed]
    [Google Scholar]
  23. Zhou Y. , Wei W. , Wang X. , Lai R. . ( 2009; ). Proposal of Sinomonas flava gen. nov., sp. nov., and description of Sinomonas atrocyanea comb. nov. to accommodate Arthrobacter atrocyaneus . . Int J Syst Evol Microbiol 59:, 259–263. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030361-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030361-0
Loading

Data & Media loading...

Supplements

Supplementary figure 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error