1887

Abstract

A novel actinomycete strain, designated CW 59, was isolated from a polluted forest soil sample in Anhui Province, China. Cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. The major fatty acids were ai-C (34.7 %), i-C (11.6 %) and ai-C (14.9 %); the predominant respiratory quinone was MK-9(H), with MK-8(H) present as a minor component. The polar lipid composition of strain CW 59 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and phosphatidylmonomethylethanolamine (minor component). The whole-cell sugars contained galactose, mannose, ribose and glucose; the major amino acids of the cell-wall were lysine, alanine and glutamic acid. The genomic DNA G+C content was 66.9 mol%. Phylogenetic analysis showed that CW 59 belonged to the genus and grouped with members of the species , , and . 16S rRNA gene sequence similarities of CW 59 to DSM 20127, CW 108, LC10 and LC13 were 99.5, 99.3, 98.2 and 98.0 %, respectively. DNA–DNA hybridization of the isolate showed relatedness values of 58.3 % (DSM 20127), 41.8 % (CW 108), 21.6 % (LC10) and 25.5 % (LC13) with its four closest neighbours. The taxonomic relationships of strains LC10 and LC13 with the genus were further clarified by means of a direct experimental comparison; results showed that strains LC10 and LC13 showed the same major fatty acid, polar lipid, cell-wall amino acid, whole-cell sugar and respiratory quinone compositions as members of the genus . Based on phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that: strain CW 59 represents a novel species of the genus , sp. nov., with CW 59 ( = CCTCC AB 207193 = KCTC 19389) as the type strain; and the type strains of and should be reclassified as comb. nov. and comb. nov., respectively. An emended description of the genus is given.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31000001)
  • , China Postdoctoral Science Foundation , (Award 20090461410)
  • , Zhejiang Province General Program Grant , (Award 2010C32024)
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2012-04-01
2020-08-07
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