1887

Abstract

The prevalence and diversity of bartonellae infecting the blood of 10 small mammal species inhabiting nine Nature Reserves of the Free State province, South Africa, was assessed using phenotypic, genotypic and phylogenetic methods. Of 86 small mammals sampled, 38 animals belonging to five different species yielded putative bartonellae. Thirty-two isolates were confirmed as bartonellae and were characterized by comparison of partial citrate synthase gene () sequences. Phylogenetic reconstructions derived from alignment of these sequences with those available for other bartonellae indicated that the South African rodent-associated isolates formed two distinct clades within the radius of the genus . One of these clades also included recognized species associated with rodents native to Eurasia but not to the New World, whereas the second clade contained exclusively isolates associated with South African rodents. Comparison of sequences delineated the isolates into a number of ecologically distinct populations and provided an indication that a combination of phylogenetics and the identification of sequence clusters in housekeeping protein-encoding genes could be developed as a key criterion in the classification of bartonellae. This study is the first to investigate wildlife-associated bartonellae in Africa, adding support to their ubiquity and broad diversity and to the paradigm that the phylogenetic positions of the species encountered today have been influenced by the geographical distribution of their hosts.

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2004-11-01
2024-12-03
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References

  1. Birtles R. J., Raoult D. 1996; Comparison of partial citrate synthase gene ( gltA ) sequences for phylogenetic analysis of Bartonella species. Int J Syst Bacteriol 46:891–897 [CrossRef]
    [Google Scholar]
  2. Birtles R. J., Harrison T. G., Molyneux D. H. 1994; Grahamella in small woodland mammals in the UK: isolation, prevalence and host specificity. Ann Trop Med Parasitol 88:317–327
    [Google Scholar]
  3. Birtles R. J., Canales J., Ventosilla P., Alvarez E., Guerra H., Llanos-Cuentas A., Raoult D., Doshi N., Harrison T. G. 1999; Survey of Bartonella species infecting intradomicillary animals in the Huayllacallán valley, Ancash, Peru, a region endemic for human bartonellosis. Am J Trop Med Hyg 60:799–805
    [Google Scholar]
  4. Bown K. J., Ellis B. A., Birtles R. J., Durden L. A., Lello J., Begon M., Bennett M. 2002; New World origins for haemoparasites infecting United Kingdom grey squirrels ( Sciurus carolinensis ), as revealed by phylogenetic analysis of bartonella infecting squirrel populations in England and the United States. Epidemiol Infect 129:647–653 [CrossRef]
    [Google Scholar]
  5. Breitschwerdt E. B., Kordick D. L. 2000; Bartonella infection in animals: carriership, reservoir potential, pathogenicity, and zoonotic potential for human infection. Clin Microbiol Rev 13:428–438 [CrossRef]
    [Google Scholar]
  6. Childs J. E., Olson J. G., Wolf A., Cohen N., Fakile Y., Rooney J. A., Bacellar F., Regnery R. L. 1995; Prevalence of antibodies to Rochalimaea species (cat-scratch disease agent) in cats. Vet Rec 136:519–520 [CrossRef]
    [Google Scholar]
  7. Cohen F. M. 2002; What are bacterial species?. Annu Rev Microbiol 56:457–487 [CrossRef]
    [Google Scholar]
  8. Droz S., Chi B., Horn E., Steigerwalt A. G., Whitney A. M., Brenner D. J. 1999; Bartonella koehlerae sp. nov., isolated from cats. J Clin Microbiol 37:1117–1122
    [Google Scholar]
  9. Ellis B. A., Regnery R. L., Beati L. 7 other authors 1999; Rats of the genus Rattus are reservoir hosts for pathogenic Bartonella species: an Old World origin for a New World disease?. J Infect Dis 180:220–224 [CrossRef]
    [Google Scholar]
  10. Fichet-Calvet E., Jomaa I., Ben Ismail R., Ashford R. W. 2000; Patterns of infection of haemoparasites in the fat sand rat, Psammomys obesus , in Tunisia, and effect on the host. Ann Trop Med Parasitol 94:55–68 [CrossRef]
    [Google Scholar]
  11. Frean J., Arndt S., Spencer D. 2002; High rate of Bartonella henselae infection in HIV-positive outpatients in Johannesburg, South Africa. Trans R Soc Trop Med Hyg 96:549–550 [CrossRef]
    [Google Scholar]
  12. Heller R., Bermond D., Delacour G., Boulouis H. J., Chomel B., Piemont Y. 1999; Distribution of Bartonella in European wild mammals. In Abstracts of the 1st International Conference on Bartonella as Emerging Pathogens Tübingen, Germany: 5–7 March 1999
    [Google Scholar]
  13. Holmberg M., Mills J. N., McGill S., Benjamin G., Ellis B. A. 2003; Bartonella infection in sylvatic small mammals in central Sweden. Epidemiol Infect 130:149–157 [CrossRef]
    [Google Scholar]
  14. Houpikian P., Raoult D. 2001; Molecular phylogeny of the genus Bartonella : what is the current knowledge?. FEMS Microbiol Lett 200:1–7 [CrossRef]
    [Google Scholar]
  15. Huelsenbeck J. P., Ronquist F. 2001; MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 [CrossRef]
    [Google Scholar]
  16. Iredell J., Blanckenberg D., Arvand M., Grauling S., Feil E. J., Birtles R. J. 2003; Characterisation of the natural population of Bartonella henselae using multi-locus sequence typing. J Clin Microbiol 41:5071–5079 [CrossRef]
    [Google Scholar]
  17. Kelly P. J., Matthewman L. A., Hayter D., Downey S., Wray K., Bryson N. R., Raoult D. 1996; Bartonella ( Rochalimaea ) henselae in southern Africa – evidence for infections in domestic cats and implications for veterinarians. J S Afr Vet Assoc 67:182–187
    [Google Scholar]
  18. Kelly P. J., Rooney J. J. A., Morsten J., Regnery R. 1998; Bartonella henselae isolated from cats in Zimbabwe. Lancet 351:1706
    [Google Scholar]
  19. Kordick D. L., Swaminathan B., Greene C. E. 11 other authors 1996; Bartonella vinsonii subsp. berkhoffii subsp. nov., isolated from dogs; Bartonella vinsonii subsp. vinsonii ; and emended description of Bartonella vinsonii . Int J Syst Bacteriol 46:704–709 [CrossRef]
    [Google Scholar]
  20. Kosoy M. Y., Regnery R. L., Tzianabos T., Marston E. L., Jones D. C., Green D., Maupin G. O., Olson J. G., Childs J. E. 1997; Distribution, diversity, and host specificity of Bartonella in rodents from the southeastern United States. Am J Trop Med Hyg 57:578–588
    [Google Scholar]
  21. Kosoy M. Y., Regnery R. L., Kosaya O. I., Childs J. E. 1999; Experimental infection of cotton rats with three naturally occurring Bartonella species. J Wildl Dis 35:275–284 [CrossRef]
    [Google Scholar]
  22. Lan R., Reeves P. R. 2001; When does a clone deserve a name? A perspective on bacterial species based on population genetics. Trends Microbiol 9:419–424 [CrossRef]
    [Google Scholar]
  23. La Scola B., Zeaiter Z., Khamis A., Raoult D. 2003; Gene sequence-based criteria for species definition in bacteriology: the Bartonella paradigm. Trends Microbiol 11:318–321 [CrossRef]
    [Google Scholar]
  24. Lawson P. A., Collins M. D. 1996; Description of Bartonella clarridgeiae sp. nov. isolated from a cat of a patient with Bartonella henselae septicemia. Med Microbiol Lett 5:64–73
    [Google Scholar]
  25. Palys T., Nakamura L. K., Cohan F. M. 1997; Discovery and classification of ecological diversity in the bacterial world: the role of DNA sequence data. Int J Syst Bacteriol 47:1145–1156 [CrossRef]
    [Google Scholar]
  26. Palys T., Berger E., Mitrica I., Nakamura L. K., Cohan F. M. 2000; Protein-coding genes as molecular markers for ecologically distinct populations: the case of two Bacillus species. Int J Syst Evol Microbiol 50:1021–1028 [CrossRef]
    [Google Scholar]
  27. Pretorius A.-M., Kelly P. J., Birtles R. J., Raoult D. 1999; Isolation of Bartonella henselae from a serologically negative cat in Bloemfontein, South Africa. J S Afr Vet Ass 70:154–155
    [Google Scholar]
  28. Raoult D., Fournier P. E., Drancourt M. 7 other authors 1996; Diagnosis of 22 new cases of Bartonella endocarditis. Ann Intern Med 125:646–652 [CrossRef]
    [Google Scholar]
  29. Raoult D., Ndihokubwayo J. B., Tissot-Dupont H., Roux V., Faugere B., Abegbinni R., Birtles R. J. 1998; Outbreak of epidemic typhus associated with trench fever in Burundi. Lancet 352:353–358 [CrossRef]
    [Google Scholar]
  30. Stevenson H. L., Bai Y., Kosoy M. Y., Montenieri J. A., Lowell J. L., Chu M. C., Gage K. L. 2003; Detection of novel Bartonella strains and Yersinia pestis in prairie dogs and their fleas (Siphonaptera: Ceratophyllidae and Pulicidae) using multiplex polymerase chain reaction. J Med Entomol 40:329–337 [CrossRef]
    [Google Scholar]
  31. Ying B., Kosoy M. Y., Maupin G. O., Tsuchiya K. R., Gage K. L. 2002; Genetic and ecologic characteristics of Bartonella communities in rodents in Southern China. Am J Trop Med Hyg 66:622–627
    [Google Scholar]
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