1887

Abstract

A Gram-negative, non-spore-forming, non-motile rod, designated MA-My1, was isolated from a tidal flat sediment of the South Sea, Korea. Strain MA-My1 grew optimally at pH 7.0–7.5, at 30 °C and with 2.0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MA-My1 clustered with CL-TF09 and S7-2, with which it exhibited 97.3 and 95.3 % 16S rRNA gene sequence similarity, respectively. Strain MA-My1 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and iso-C 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 30.6 mol% and DNA–DNA relatedness between strain MA-My1 and JCM 13034 was 6.3±0.8 %. The differential phenotypic properties, together with phylogenetic and genotypic distinctiveness, distinguished strain MA-My1 from the members of the genus . On the basis of the data presented, strain MA-My1 is considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is MA-My1 ( = KCTC 23499  = CCUG 60022).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030320-0
2012-02-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/2/420.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030320-0&mimeType=html&fmt=ahah

References

  1. Asker D. , Beppu T. , Ueda K. . ( 2007; ). Zeaxanthinibacter enoshimensis gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae, isolated from seawater off Enoshima Island, Japan. . Int J Syst Evol Microbiol 57:, 837–843. [CrossRef] [PubMed]
    [Google Scholar]
  2. Baumann P. , Baumann L. . ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P. , Stolp H. , Trüper H. G. , Balows A. , Schlegel H. G. . . Berlin:: Springer;.
    [Google Scholar]
  3. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef] [PubMed]
    [Google Scholar]
  5. Choi D. H. , Cho B. C. . ( 2006; ). Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. . Int J Syst Evol Microbiol 56:, 771–776. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cohen-Bazire G. , Sistrom W. R. , Stanier R. Y. . ( 1957; ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49:, 25–68. [CrossRef]
    [Google Scholar]
  7. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  8. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  9. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  10. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  11. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  12. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  13. Park S. , Kang S.-J. , Oh T.-K. , Yoon J.-H. . ( 2010; ). Lutibacter maritimus sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 60:, 610–614. [CrossRef] [PubMed]
    [Google Scholar]
  14. Reichenbach H. . ( 1992; ). The order Cytophagales. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  15. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  16. Staley J. T. . ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  17. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  18. Teather R. M. , Wood P. J. . ( 1982; ). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43:, 777–780.[PubMed]
    [Google Scholar]
  19. Yoon J.-H. , Kim H. , Kim S.-B. , Kim H.-J. , Kim W. Y. , Lee S. T. , Goodfellow M. , Park Y.-H. . ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  20. Yoon J.-H. , Lee S. T. , Park Y.-H. . ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef] [PubMed]
    [Google Scholar]
  21. Yoon J.-H. , Kim I.-G. , Shin D.-Y. , Kang K. H. , Park Y.-H. . ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53:, 53–57. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030320-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030320-0
Loading

Data & Media loading...

Supplements

Supplementary Fig. S1 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error