1887

Abstract

A novel haloalkaliphilic archaeon, strain 8W8, was isolated from Lake Zabuye, on the Tibetan Plateau, China. On the basis of 16S rRNA gene sequence analysis, strain 8W8 was shown to belong to the genus and was related to (96·7 % sequence similarity), (96·0 %), (95·4 %) and (95·3 %). The phylogenetic distance from any species within the other genera of was lower than 90 %. The major polar lipids of strain 8W8 were CC and CC derivatives of phosphatidylglycerol phosphate and phosphatidylglycerol phosphate methyl ester. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 8W8 from the eight species with validly published names. Therefore, strain 8W8 represents a novel species, for which the name sp. nov. is proposed, with the type strain 8W8 (=AS 1.3239=JCM 11889).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.03032-0
2004-07-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/4/ijs541213.html?itemId=/content/journal/ijsem/10.1099/ijs.0.03032-0&mimeType=html&fmt=ahah

References

  1. Franzmann, P. D., Stackebrandt, E., Sanderson, K., Volkman, J. K., Cameron, D. E., Stevenson, P. L., McMeekin, T. A. & Burton, H. R. ( 1988; ). Halobacterium lacusprofundi sp. nov., a halophilic bacterium isolated from Deep Lake, Antarctica. Syst Appl Microbiol 11, 20–27.[CrossRef]
    [Google Scholar]
  2. Grant, W. D., Kamekura, M., McGenity, T. J. & Ventosa, A. ( 2001; ). Order I. Halobacteriales. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 294–334. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  3. Holland, H. D., Smith, G. I., Jannasch, H. W., Dickson, A. G., Zheng, M. & Ding, T. ( 1991; ). Lake Zabuye and the climatic history of the Tibetan Plateau. Geowissenschaften 9, 37–44 (in German).
    [Google Scholar]
  4. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  5. Kamekura, M. & Dyall-Smith, M. L. ( 1995; ). Taxonomy of the family Halobacteriaceae and the description of two new genera Halorubrobacterium and Natrialba. J Gen Appl Microbiol 41, 333–350.[CrossRef]
    [Google Scholar]
  6. Kamekura, M., Dyall-Smith, M. L., Upasani, V., Ventosa, A. & Kates, M. ( 1997; ). Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba, and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47, 853–857.[CrossRef]
    [Google Scholar]
  7. Lizama, C., Monteoliva-Sanchez, M., Suarez-Garcia, A., Rosello-Mora, R., Aguilera, M., Campos, V. & Ramos-Cormenzana, A. ( 2002; ). Halorubrum tebenquichense sp. nov., a novel halophilic archaeon isolated from the Atacama Saltern, Chile. Int J Syst Evol Microbiol 52, 149–155.
    [Google Scholar]
  8. McGenity, T. J. & Grant, W. D. ( 1993; ). The haloalkaliphilic archaeon (archaebacterium) Natronococcus occultus represents a distinct lineage within the Halobacteriales, most closely related to the other haloalkaliphilic lineage (Natronobacterium). Syst Appl Microbiol 16, 239–243.
    [Google Scholar]
  9. McGenity, T. J. & Grant, W. D. ( 1995; ). Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18, 237–243.[CrossRef]
    [Google Scholar]
  10. Mwatha, W. E. & Grant, W. D. ( 1993; ). Natronobacterium vacuolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. Int J Syst Bacteriol 43, 401–404.[CrossRef]
    [Google Scholar]
  11. Oren, A. ( 1983; ). Halobacterium sodomense sp. nov., a Dead Sea halobacterium with an extremely high magnesium requirement. Int J Syst Bacteriol 33, 381–386.[CrossRef]
    [Google Scholar]
  12. Oren, A. & Ventosa, A. ( 1996; ). A proposal for the transfer of Halorubrobacterium distributum and Halorubrobacterium coriense to the genus Halorubrum as Halorubrum distributum comb. nov. and Halorubrum coriense comb. nov., respectively. Int J Syst Bacteriol 46, 1180.[CrossRef]
    [Google Scholar]
  13. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  14. Petter, H. F. M. ( 1931; ). On bacteria of salted fish. Proc K Ned Akad Wet Amsterdam 34, 1417–1423 (in Dutch).
    [Google Scholar]
  15. Ross, H. N. M., Collins, M. D., Tindall, B. J. & Grant, W. D. ( 1981; ). A rapid procedure for the detection of archaebacterial lipids in halophilic bacteria. J Gen Microbiol 123, 75–80.
    [Google Scholar]
  16. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position-specific gap penalities and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  17. Tindall, B. J., Mills, A. A. & Grant, W. D. ( 1980; ). An alkalophilic red halophilic bacterium with a low magnesium requirement from a Kenyan soda lake. J Gen Microbiol 116, 257–260.
    [Google Scholar]
  18. Tomlinson, G. A. & Hochstein, L. I. ( 1976; ). Halobacterium saccharovorum sp. nov., a carbohydrate-metabolizing extremely halophilic bacterium. Can J Microbiol 22, 587–591.[CrossRef]
    [Google Scholar]
  19. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  20. Zvyagintseva, I. S. & Tarasov, A. L. ( 1987; ). Extreme halophilic bacteria from saline soils. Mikrobiologiya 56, 839–844 (in Russian).
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.03032-0
Loading
/content/journal/ijsem/10.1099/ijs.0.03032-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error