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Abstract

A novel extremely thermophilic, hydrogen- and sulfur-oxidizing bacterium, designated strain IBSK3, was isolated from a coastal hot spring in Ibusuki, Kagoshima Prefecture, Japan. The cells were motile, straight to slightly curved rods (1·2–3·0 μm long and 0·3–0·4 μm wide). Strain IBSK3 was an obligate chemolithoautotroph growing by respiratory nitrate reduction with H, forming NO as an end product. Low concentrations of O (0·4–7·7 %, v/v; optimum 2·0 %, v/v) could serve as an alternative electron acceptor to growth. In addition, strain IBSK3 was able to utilize elemental sulfur as a sole electron donor with either nitrate or low concentrations of O as an electron acceptor. Growth was observed between 55 and 77·5 °C (optimum 75 °C; 2 h doubling time), pH 5·5 and 8·3 (optimum pH 6·5–7·0), and in the presence of 0·5 and 4·0 % NaCl (optimum 2·0 %). The G+C content of the genomic DNA was 49·2 mol%. On the basis of 16S rRNA gene sequence analysis, strain IBSK3 belonged to the family , but it only demonstrated a distant phylogenetic relationship with any recognized species within the family (sequence similarity was less than 92 %). On the basis of the physiological and molecular characteristics of the novel isolate, a new genus and novel species are proposed: the type strain of gen. nov., sp. nov. is IBSK3 (=JCM 12173=ATCC BAA-821).

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2004-11-01
2019-10-14
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vol. , part 6, pp. 2079–2084

A phase-contrast micrograph of strain IBSK3 cells showing filamentous morphology and graphs showing the effects of temperature, pH, NaCl concentration and O concentration in the gas phase on the growth of strain IBSK3 are available to download. [PDF](381KB)



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