1887

Abstract

Twelve strains of psychrophilic bacteria were isolated from cyanobacterial mat samples collected from various water bodies in the McMurdo Dry Valley region of Antarctica. All the isolates were Gram-negative, non-motile, coccoid, psychrophilic, halotolerant bacteria and had C 7, C 8 and C 9 as the major fatty acids, ubiquinone-8 as the respiratory quinone and DNA G+C content of 41–46 mol%. Based on these characteristics, the isolates were assigned to the genus . Based on their SDS-PAGE profiles, the 12 isolates could be categorized into three groups. Six isolates of Group I were identified as representing strains of . However, using detailed phenotypic and chemotaxonomic characteristics and phylogenetic analysis based on their 16S rRNA gene sequences, strain CMS 39, the only strain from Group II, and strain CMS 56, a representative strain of Group III, were different from each other and from all recognized species of . Therefore, it is proposed to classify CMS 39 (=DSM 15337=MTCC 4208) and CMS 56 (=DSM 15339=MTCC 4386) as representing the type strains of novel species of , for which the names sp. nov. and sp. nov., respectively, are proposed.

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2005-03-01
2019-09-18
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References

  1. Bowman, J. P., Cavanagh, J., Austin, J. J. & Sanderson, K. ( 1996; ). Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46, 841–848.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P., Nichols, D. S. & McMeekin, T. A. ( 1997; ). Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic sea ice. Syst Appl Microbiol 20, 209–215.[CrossRef]
    [Google Scholar]
  3. Bozal, N., Montes, M. J., Tudela, E. & Guinea, J. ( 2003; ). Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov. Int J Syst Evol Microbiol 53, 1093–1100.[CrossRef]
    [Google Scholar]
  4. Denner, E. B. M., Mark, B., Busse, H.-J., Turkiewicz, M. & Lubitz, W. ( 2001; ). Psychrobacter proteolyticus sp. nov., a psychrophilic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease. Syst Appl Microbiol 24, 44–53.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  6. Juni, E. ( 1991; ). The genus Psychrobacter. In The Prokaryotes, pp. 3241–3246. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  7. Juni, E. & Heym, G. A. ( 1986; ). Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 46, 388–391.
    [Google Scholar]
  8. Kämpfer, P., Albrecht, A., Buczolits, S. & Busse, H.-J. ( 2002; ). Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. Syst Appl Microbiol 25, 31–36.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  11. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–147. Edited by E. Stackebrandt & M. Goodfellow. New York: Wiley.
  12. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important Bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  13. Maruyama, A., Honda, D., Yamamoto, H., Kitamura, K. & Higashihara, T. ( 2000; ). Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep sea species Psychrobacter pacificensis sp. nov. Int J Syst Evol Microbiol 50, 835–846.[CrossRef]
    [Google Scholar]
  14. Matsumoto, G. I. ( 1993; ). Geochemical features of the McMurdo Dry Valley lakes, Antarctica. Physical and biogeochemical processes in Antarctic Lakes. Antarct Res Ser 49, 95–118.
    [Google Scholar]
  15. Matsumoto, G. I., Ohtani, S. & Hiroto, K. ( 1993; ). Biogeochemical features of hydrocarbons in cyanobacterial mats from the McMurdo Dry Valley, Antarctica. Proc NIPR Symp Polar Biol 6, 98–105.
    [Google Scholar]
  16. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S. ( 2000; ). Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef]
    [Google Scholar]
  17. Reddy, G. S. N., Prakash, J. S. S., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S. ( 2002a; ). Arthrobacter roseus sp. nov., a psychrotolerant bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52, 1017–1021.[CrossRef]
    [Google Scholar]
  18. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S. ( 2002b; ). Planococcus antarcticus and Planococcus psychrophilus sp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–261.[CrossRef]
    [Google Scholar]
  19. Reddy, G. S. N., Raghavan, P. U. M., Sarita, N. B., Prakash, J. S. S., Nagesh, N., Delille, D. & Shivaji, S. ( 2003; ). Halomonas glaciei sp. nov. isolated from fast ice of Adelie Land, Antarctica. Extremophiles 7, 55–61.
    [Google Scholar]
  20. Romanenko, L. A., Schumann, P., Rhode, M., Lysenko, A. M., Mikhailov, V. V. & Stackebrandt, E. ( 2002; ). Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles isolated from marine environments. Int J Syst Evol Microbiol 52, 1291–1297.[CrossRef]
    [Google Scholar]
  21. Rossau, R., van Landschoot, A., Gillis, M. & De Ley, J. ( 1991; ). Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and Psychrobacter and related organisms. Int J Syst Bacteriol 41, 310–319.[CrossRef]
    [Google Scholar]
  22. Shivaji, S., Rao, N. S., Saisree, L., Sheth, V., Reddy, G. S. N. & Bhargava, P. M. ( 1989; ). Isolation and identification of Pseudomonas spp. from Schirmacher Oasis, Antarctica. Appl Environ Microbiol 55, 767–770.
    [Google Scholar]
  23. Shivaji, S., Bhanu, N. V. & Aggarwal, R. K. ( 2000; ). Identification of Yersinia pestis as the causative organism of plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189, 247–252.[CrossRef]
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  25. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  26. Vela, A. I., Collins, M. D., Latre, M. V., Mateos, A., Moreno, M. A., Hutson, R., Dominguez, L. & Fernandez-Garayzabal, J. F. ( 2003; ). Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int J Syst Evol Microbiol 53, 415–419.[CrossRef]
    [Google Scholar]
  27. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
  28. Yumoto, I., Hirota, K., Sogabe, Y., Nodasaka, Y., Yokota, Y. & Hoshino, T. ( 2003; ). Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 53, 1985–1989.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 757–762

Tables giving additional data relating to fatty acid profiles, phospholipids, 16S rRNA gene sequence similarity and DNA–DNA relatedness are available to download. [PDF](108KB)



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