A Gram-staining-negative, non-spore-forming, facultatively aerobic, non-motile, rod-shaped bacterial strain, BR-3T, was isolated from a tidal flat on the western coast of Korea, and subjected to a polyphasic study. Strain BR-3T grew optimally at 25 °C, at pH 6.5–7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-3T fell within the clade comprising species of the genus Mucilaginibacter, joining the type strain of Mucilaginibacter rigui, with which it exhibited highest 16S rRNA gene sequence similarity (98.2 %). 16S rRNA gene sequence similarity values between strain BR-3T and the type strains of the other species of the genus Mucilaginibacter were in the range 93.8–95.9 %. A mean DNA–DNA relatedness value between strain BR-3T and M. rigui KCTC 12534T was 21 %. Strain BR-3T contained MK-7 as the predominant menaquinone and C16 : 1ω7c and/or iso-C15 : 0 2-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The DNA G+C content was 49.8 mol%. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain BR-3T demonstrated that this strain is separate from M. rigui as well as the other species of the genus Mucilaginibacter. On the basis of the data presented, strain BR-3T is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter lutimaris sp. nov. is proposed. The type strain is BR-3T ( = KCTC 23461T = CCUG 60742T).
Published Online:
Funding
This study was supported by the:
Program for Collection, Management and Utilization of Biological Resources
(Award 11-2008-00-002-00)
21C Frontier Program of Microbial Genomics and Applications
(Award MG05-0401-2-0)
Ministry of Education, Science and Technology (MEST)
BaikK. S.,
ParkS. C.,
KimE. M.,
LimC. H.,
SeongC. N.2010; Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter
. Int J Syst Evol Microbiol 60:134–139 [View Article][PubMed]
BowmanJ. P.2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
BrunsA.,
RohdeM.,
Berthe-CortiL.2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
JeonY.,
LeeS.-S.,
ChungB. S.,
KimJ. M.,
BaeJ.-W.,
ParkS. K.,
JeonC. O.2009; Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. Int J Syst Evol Microbiol 59:1451–1454 [View Article][PubMed]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
PankratovT. A.,
TindallB. J.,
LiesackW.,
DedyshS. N.2007; Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57:2349–2354 [View Article][PubMed]
ReichenbachH.1992; The order Cytophagales
. In The Prokaryotes, 2nd edn. vol. 4 pp. 3631–3675 Edited by
BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK. H.
New York: Springer; [CrossRef]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.
other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
YoonJ.-H.,
KimH.,
KimS.-B.,
KimH.-J.,
KimW. Y.,
LeeS. T.,
GoodfellowM.,
ParkY.-H.1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
YoonJ.-H.,
LeeS. T.,
ParkY.-H.1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
YoonJ.-H.,
KimI.-G.,
ShinD.-Y.,
KangK. H.,
ParkY.-H.2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]