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Abstract

A Gram-staining-negative, non-spore-forming, facultatively aerobic, non-motile, rod-shaped bacterial strain, BR-3, was isolated from a tidal flat on the western coast of Korea, and subjected to a polyphasic study. Strain BR-3 grew optimally at 25 °C, at pH 6.5–7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-3 fell within the clade comprising species of the genus , joining the type strain of , with which it exhibited highest 16S rRNA gene sequence similarity (98.2 %). 16S rRNA gene sequence similarity values between strain BR-3 and the type strains of the other species of the genus were in the range 93.8–95.9 %. A mean DNA–DNA relatedness value between strain BR-3 and . KCTC 12534 was 21 %. Strain BR-3 contained MK-7 as the predominant menaquinone and Cω7 and/or iso-C 2-OH and iso-C as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The DNA G+C content was 49.8 mol%. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain BR-3 demonstrated that this strain is separate from . as well as the other species of the genus . On the basis of the data presented, strain BR-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BR-3 ( = KCTC 23461  = CCUG 60742).

Funding
This study was supported by the:
  • , Program for Collection, Management and Utilization of Biological Resources , (Award 11-2008-00-002-00)
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award MG05-0401-2-0)
  • , Ministry of Education, Science and Technology (MEST)
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2012-03-01
2020-08-09
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