1887

Abstract

Two actinobacterial strains, JR-43 and JR-4, were isolated from bamboo () rhizosphere soil. The isolates produced grey aerial mycelium and a yellow soluble pigment on ISP 4. Microscopic observation revealed that strains JR-43 and JR-4 produced spore chains with spiny surfaces. Both isolates had antibacterial activity against plant-pathogenic bacteria, such as LMG 568 and pv. LMG 905. The isolates contained iso-C, iso-C, anteiso-C and iso-C as the major fatty acids and MK-9(H) and MK-9(H) as the major isoprenoid quinones. Phylogenetic analysis of the 16S rRNA gene sequences of strains JR-43 and JR-4 showed that they grouped within cluster II and had highest sequence similarity to NBRC 16668 and NBRC 12813 (both 98.2 % 16S rRNA gene sequence similarity). DNA–DNA relatedness between strain JR-43 and NBRC 16668 and NBRC 12813 ranged from 31.42 to 42.92 %. Based on DNA–DNA relatedness and morphological and phenotypic data, strains JR-43 and JR-4 could be distinguished from the type strains of phylogenetically related species. They are therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JR-43 ( = KACC 15079  = NBRC 107863). Strain JR-43 ( = KACC 15078  = NBRC 107864) is a reference strain.

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2012-04-01
2019-12-13
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