1887

Abstract

A pink, aerobic, facultatively methylotrophic, motile, Gram-negative rod, designated Gh-105, was isolated from the phyllosphere of cotton from Coimbatore (Tamilnadu, India). 16S rRNA gene sequence analysis showed clearly that the isolate belonged to the cluster. Strain Gh-105 was most closely related to AR27 (99 % 16S rRNA gene sequence similarity) and 5317S-33 (97.5 %). The isolate grew with C compounds such as methanol and dichloromethane, but not with formaldehyde, formate, methylamine, trimethylamine or methane, as sole carbon sources and carried , which encodes methanol dehydrogenase and supports methylotrophic metabolism. The major fatty acid was Cω7 and the G+C content of the genomic DNA was 64.2 mol%. Physiological and biochemical data and DNA–DNA relatedness with KACC 12195 and KACC 11765 revealed clear phenotypic and genotypic differences. For this reason, we propose that strain Gh-105 ( = CCM 7572  = NRRL B-51692) represents the type strain of a novel species, with the name sp. nov.

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2012-01-01
2019-12-05
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