RT Journal Article SR Electronic(1) A1 Yumoto, Isao A1 Hirota, Kikue A1 Yamaga, Shingo A1 Nodasaka, Yoshinobu A1 Kawasaki, Tsuneshirou A1 Matsuyama, Hidetoshi A1 Nakajima, KenjiYR 2004 T1 Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids JF International Journal of Systematic and Evolutionary Microbiology, VO 54 IS 6 SP 1997 OP 2001 DO https://doi.org/10.1099/ijs.0.03014-0 PB Microbiology Society, SN 1466-5034, AB In a screening campaign to isolate strains with the ability to remove the bad smell associated with animal faeces, strain MA001T was isolated from a soil sample obtained from Shizuoka prefecture, Japan. The isolate grew at pH 6–9 but not at pH 10. Cells were Gram-positive, straight rods with peritrichous flagella and produced ellipsoidal spores. The isolate was positive for catalase and oxidase tests but negative for indole production, deamination of phenylalanine and H2S production. The isolate did not produce acid from any carbohydrates tested and could not grow in more than 2 % NaCl. The DNA G+C content was 39·4 mol%. The cellular fatty acids profile consisted of significant amount of C15 branched-chain fatty acids, iso-C15 : 0 and anteiso-C15 : 0. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain MA001T was closely related to Bacillus simplex and Bacillus psychrosaccharolyticus. DNA–DNA hybridization revealed a low relatedness of the isolate to several phylogenetically close neighbours (less than 9 %). On the basis of the phenotypic characteristics observed, phylogenetic data based on 16S rRNA gene sequencing and DNA–DNA relatedness data, it is concluded that the isolate should be classified as representing a novel species, for which the name Bacillus asahii is proposed. The type strain is MA001T (=JCM 12112T=NCIMB 13969T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.03014-0