RT Journal Article SR Electronic(1) A1 Butler, Margaret K. A1 Fuerst, John A.YR 2004 T1 Comparative analysis of ribonuclease P RNA of the planctomycetes JF International Journal of Systematic and Evolutionary Microbiology, VO 54 IS 4 SP 1333 OP 1344 DO https://doi.org/10.1099/ijs.0.03013-0 PB Microbiology Society, SN 1466-5034, AB The planctomycetes, order Planctomycetales, are a distinct phylum of domain Bacteria. Genes encoding the RNA portion of ribonuclease P (RNase P) of some planctomycete members were sequenced and compared with existing database planctomycete sequences. rnpB gene sequences encoding RNase P RNA were generated by a conserved primer PCR strategy for Planctomyces brasiliensis, Planctomyces limnophilus, Pirellula marina, Pirellula staleyi strain ATCC 35122, Isosphaera pallida, one other Isosphaera strain, Gemmata obscuriglobus and three other strains of the Gemmata group. These sequences were aligned against reference bacterial sequences and secondary structures of corresponding RNase P RNAs deduced by a comparative approach. P12 helices were found to be highly variable in length, as were helices P16.1 and P19, when present. RNase P RNA secondary structures of Gemmata isolates were found to have unusual features relative to other planctomycetes, including a long P9 helix and an insert in the P13 helix not found in any other member of domain Bacteria. These unique features are consistent with other unusual properties of this genus, distinguishing it from other bacteria. Phylogenetic analyses indicate that relationships between planctomycetes derived from RNase P RNA are consistent with 16S rRNA-based analyses., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.03013-0