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Volume 54, Issue 4

Comparative analysis of ribonuclease P RNA of the planctomycetes Free

Abstract

The planctomycetes, order , are a distinct phylum of domain . Genes encoding the RNA portion of ribonuclease P (RNase P) of some planctomycete members were sequenced and compared with existing database planctomycete sequences. gene sequences encoding RNase P RNA were generated by a conserved primer PCR strategy for , , , strain ATCC 35122, , one other strain, and three other strains of the group. These sequences were aligned against reference bacterial sequences and secondary structures of corresponding RNase P RNAs deduced by a comparative approach. P12 helices were found to be highly variable in length, as were helices P16.1 and P19, when present. RNase P RNA secondary structures of isolates were found to have unusual features relative to other planctomycetes, including a long P9 helix and an insert in the P13 helix not found in any other member of domain . These unique features are consistent with other unusual properties of this genus, distinguishing it from other bacteria. Phylogenetic analyses indicate that relationships between planctomycetes derived from RNase P RNA are consistent with 16S rRNA-based analyses.

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2004-07-01
2024-04-19
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Comparative analysis of ribonuclease P RNA of the planctomycetes
Int J Syst Evol Microbiol 54, 1333 (2004); https://doi.org/10.1099/ijs.0.03013-0
/content/journal/ijsem/10.1099/ijs.0.03013-0
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