1887

Abstract

A thermo-acidophilic Gram-positive bacterium, strain CsHg2, which grows aerobically at 35–65 °C (optimum 55 °C) and at pH 2·0–6·0 (optimum 4·0), was isolated from a geothermal pool located in Coso Hot Springs in the Mojave Desert, California, USA. Phylogenetic analysis of 16S rRNA gene sequences showed that this bacterium was most closely related to the type strains of (97·8 % identity) and (96·9 %), three Japanese strains denoted as UZ-1, KHA-31 and MIH 332 (96·1–96·5 %) and genomic species FR-6 (96·3 %). Phenotypic characteristics including temperature and pH optima, G+C composition, acid production from a variety of carbon sources and sensitivity to different metal salts distinguished CsHg2 from , and FR-6. The cell lipid membrane was composed mainly of -cyclohexyl fatty acid, consistent with membranes from other species. Very low DNA–DNA hybridization values between CsHg2 and the type strains of indicate that CsHg2 represents a distinct species. On the basis of these results, the name sp. nov. is proposed for this organism. The type strain is CsHg2 (ATCC BAA-915=DSM 16176).

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2004-09-01
2020-01-23
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References

  1. Albuquerque, L., Rainey, F. A., Chung, A. P., Sunna, A., Nobre, M. F., Grote, R., Antranikian, G. & da Costa, M. S. ( 2000; ). Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores. Int J Syst Evol Microbiol 50, 451–457.[CrossRef]
    [Google Scholar]
  2. Allen, M. B. ( 1959; ). Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch Mikrobiol 32, 270–277.[CrossRef]
    [Google Scholar]
  3. Bligh, E. G. & Dyer, W. ( 1959; ). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.[CrossRef]
    [Google Scholar]
  4. Brock, T., Brock, K., Belly, R. & Weiss, R. ( 1972; ). Sulfolobus: a new genus of sulfur oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol 84, 54–68.[CrossRef]
    [Google Scholar]
  5. De Long, E. F. ( 1992; ). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89, 5685–5689.[CrossRef]
    [Google Scholar]
  6. Deinhard, G., Saar, J., Krischke, W. & Poralla, K. ( 1987; ). Bacillus cycloheptanicus sp. nov., a new thermophile containing ω-cycloheptane fatty acids. Syst Appl Microbiol 10, 47–53.[CrossRef]
    [Google Scholar]
  7. Gonzalez, J. M. & Saiz-Jimenez, C. ( 2002; ). A fluorometric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef]
    [Google Scholar]
  8. Goto, K., Matsubara, H., Mochida, K., Matsumura, T., Hara, Y., Niwa, M. & Yamasato, K. ( 2002; ). Alicyclobacillus herbarius sp. nov., a novel bacterium containing ω-cycloheptane fatty acids, isolated from herbal tea. Int J Syst Evol Microbiol 52, 109–113.
    [Google Scholar]
  9. Goto, K., Mochida, K., Asahara, M., Suzuki, M., Kasai, H. & Yokota, A. ( 2003; ). Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol 53, 1537–1544.[CrossRef]
    [Google Scholar]
  10. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  11. Haseltine, C., Montalvo-Rodriguez, R., Carl, A., Bini, E. & Blum, P. ( 1999; ). Extragenic pleiotropic mutations that repress glycosyl hydrolase expression in the hyperthermophilic archaeon Sulfolobus solfataricus. Genetics 152, 1353–1361.
    [Google Scholar]
  12. Haseltine, C., Hill, T., Montalvo-Rodriguez, R., Kemper, S., Shand, R. & Blum, P. ( 2001; ). Secreted euryarchaeal microhalocins kill hyperthermophilic crenarchaea. J Bacteriol 183, 287–291.[CrossRef]
    [Google Scholar]
  13. Hiraishi, A., Inagaki, K., Tanimoto, Y., Iwasaki, M., Kishimoto, N. & Tanaka, H. ( 1997; ). Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot springs in Japan. J Gen Appl Microbiol 43, 295–304.[CrossRef]
    [Google Scholar]
  14. Hucker, G. & Conn, H. ( 1923; ). Methods of Gram Staining. New York State Agricultural Experiment Station Technical Bulletin no. 93.
  15. Kates, M. ( 1986; ). Techniques of lipidology: isolation, analysis and identification of lipids. In Laboratory Techniques in Biochemistry and Molecular Biology, vol. 3, part 2. Edited by R. H. Burdon & P. H. van Kippenberg. New York: Elsevier.
  16. Kumar, S., Tamura, K., Jakobsen, I. & Nei, M. ( 2001; ). mega2: Molecular Evolutionary Genetics Analysis software. Tempe, AZ: Arizona State University.
  17. Matsubara, H., Goto, K., Matsumura, T., Mochida, K., Iwaki, M., Niwa, M. & Yamasoto, K. ( 2002; ). Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, ω-alicyclic fatty acid-containing bacterium isolated from acidic beverages. Int J Syst Evol Microbiol 52, 1681–1685.[CrossRef]
    [Google Scholar]
  18. Oshima, M. & Ariga, T. ( 1975; ). Omega-cyclohexyl fatty acids in acidophilic thermophilic bacteria. Studies on their presence, structure, and biosynthesis using precursors labeled with stable isotopes and radioisotopes. J Biol Chem 250, 6963–6968.
    [Google Scholar]
  19. Partridge, J., King, J., Krska, J., Rockabrand, D. & Blum, P. ( 1993; ). Cloning, heterologous expression, and characterization of the Erysipelothrix rhusiopathiae DnaK protein. Infect Immun 61, 411–417.
    [Google Scholar]
  20. Rolfsmeier, M. & Blum, P. ( 1995; ). Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus. J Bacteriol 177, 482–485.
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Tsuruoka, N., Isono, Y., Shida, O., Hemmi, H., Nakayama, T. & Nishino, T. ( 2003; ). Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. Int J Syst Evol Microbiol 53, 1081–1084.[CrossRef]
    [Google Scholar]
  23. Urbance, J. W., Bratina, B. J., Stoddard, S. F. & Schmidt, T. M. ( 2001; ). Taxonomic characterization of Ketogulonigenium vulgare gen. nov., sp. nov. and Ketogulonigenium robustum sp. nov., which oxidize l-sorbose to 2-keto-l-gulonic acid. Int J Syst Evol Microbiol 51, 1059–1070.[CrossRef]
    [Google Scholar]
  24. Wayne, L., Brenner, D., Colwell, R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  25. White, D., Davis, W., Nickels, J., King, J. & Bobbie, R. ( 1979; ). Determination of the sedimentary microbial biomass by extractable liquid phosphate. Oecologia 40, 51–62.[CrossRef]
    [Google Scholar]
  26. Wisotzkey, J. D., Jurtshuk, P., Jr, Fox, G. E., Deinhard, G. & Poralla, K. ( 1992; ). Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42, 263–269.[CrossRef]
    [Google Scholar]
  27. Yu, Q., Lui, B., Zhang, J. & Huang, Z. ( 1989; ). Location of double bonds in fatty acids of fish oil and rat testis lipids. Gas chromatography-mass spectrometry of the oxazoline derivatives. Lipids 24, 79–83.[CrossRef]
    [Google Scholar]
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