1887

Abstract

The taxonomic standing of and was investigated using a polyphasic approach. Sixteen representatives of both taxa were found to be phenotypically similar and shared more than 70 % DNA–DNA relatedness (76–100 %), which reinforces the conclusions of previous studies in which and were considered to be one single species. However, the results of protein profiling, BOX-PCR fingerprinting, Fluorescent Amplified Fragment Length Polymorphism (FAFLP), and and gene sequence analysis demonstrate that representatives of and constitute two clearly separated subgroups; this subdivision was also phenotypically supported based on the ability to grow in milk. Given the fact that Meile . 1997 has to be considered as a junior synonym of (Mitsuoka 1969) Scardovi and Trovatelli 1974 , our data indicate that the latter species should be split into two new subspecies, i.e. subsp. subsp. nov. (type strain R101-8=LMG 10508=ATCC 25527=DSM 20104=JCM 1190) and subsp. subsp. nov. (type strain UR1=LMG 18314=DSM 10140=JCM 10602).

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2004-07-01
2024-12-14
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References

  1. Cai Y., Matsumoto M., Benno Y. 2000; Bifidobacterium lactis Meile et al . 1997 is a subjective synonym of Bifidobacterium animalis (Mitsuoka 1969 Scardovi and Trovatelli 1974). Microbiol Immunol 44:815–820 [CrossRef]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid- deoxyribonucleic acid hybridization wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA-DNA hybridisation method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
    [Google Scholar]
  4. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. 1992 International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Lauer E., Kandler O. 1983; DNA-DNA homology, murein types and enzyme patterns in the type strains of the genus Bifidobacterium . Syst Appl Microbiol 4:42–64 [CrossRef]
    [Google Scholar]
  6. Lawrence J. G., Hartl D. L., Ochman H. 1991; Molecular considerations in the evolution of bacterial genes. J Mol Evol 33:241–250 [CrossRef]
    [Google Scholar]
  7. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  8. Masco L., Huys G., Gevers D., Verbrugghen L., Swings J. 2003; Identification of Bifidobacterium species using rep-PCR fingerprinting. Syst Appl Microbiol 26:557–563 [CrossRef]
    [Google Scholar]
  9. Meile L., Ludwig W., Rueger U., Gut C., Kaufmann P., Dasen G., Wenger S., Teuber T. 1997; Bifidobacterium lactis sp. nov., a moderately oxygen tolerant species isolated from fermented milk. Syst Appl Microbiol 20:57–64 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  12. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organisms protein fingerprints. In Modern Microbial Methods , Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  13. Prasad J., Gill H., Smart J., Gopal P. K. 1998; Selection and characterization of Lactobacillus and Bifidobacterium strains for use as probiotics. Int Dairy J 8:993–1002 [CrossRef]
    [Google Scholar]
  14. Scardovi V., Trovatelli L. D. 1974; Bifidobacterium animalis (Mitsuoka) comb. nov. and the “ minimum ” and “ subtile ” groups of new bifidobacteria found in sewage. Int J Syst Bacteriol 24:21–28 [CrossRef]
    [Google Scholar]
  15. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2001; Genomic diversity amongst Vibrio isolates from different sources determined by Fluorescent Amplified Fragment Length Polymorphism. Syst Appl Microbiol 24:520–538 [CrossRef]
    [Google Scholar]
  16. Ventura M., Elli M., Reniero M., Zink R. 2001; Molecular microbial analysis of Bifidobacterium isolates from different environments by species-specific amplified ribosomal DNA restriction analysis (ARDRA). FEMS Microbiol Ecol 36:113–121 [CrossRef]
    [Google Scholar]
  17. Ventura M., Zink R. 2002; Rapid identification, differentiation, and proposed new taxonomic classification of Bifidobacterium lactis . Appl Environ Microbiol 68:6429–6434 [CrossRef]
    [Google Scholar]
  18. Ventura M., Zink R. 2003; Comparative sequence analysis of the tuf and recA genes and restriction fragment length polymorphism of the internal transcribed spacer region sequences supply additional tools for discriminating Bifidobacterium lactis from Bifidobacterium animalis . Appl Environ Microbiol 69:7517–7522 [CrossRef]
    [Google Scholar]
  19. Zhu L., Li W., Dong X. 2003; Species identification of genus Bifidobacterium based on partial HSP60 gene sequences and proposal of Bifidobacterium thermacidophilum subsp. porcinum subsp. nov. Int J Syst Evol Microbiol 53:1619–1623 [CrossRef]
    [Google Scholar]
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