1887

Abstract

The taxonomic position of a polycyclic-aromatic-hydrocarbon-degrading bacterium, strain 17A3, isolated from contaminated soil was determined using a combination of phenotypic and genotypic properties. The isolate showed phenotypic properties that were diagnostic for species of the genus . Comparative 16S rRNA gene sequence analysis assigned 17A3 to the 16S rRNA gene subgroup that contains , , and , but it could clearly be distinguished from these species using a combination of physiological, chemotaxonomic markers and internal rRNA gene spacer analyses. The data showed that strain 17A3 (=DSM 44605=NRRL B-24244) merits recognition as the type strain of a novel species of the genus . The name sp. nov. is proposed for the species because of its ability to use pyrene as a sole source of carbon and energy.

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2004-11-01
2019-12-12
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References

  1. Bell, K., Philip, J. C., Aw, D. W. J. & Christofi, N. ( 1998; ). The genus Rhodococcus. J Appl Microbiol 85, 195–210.[CrossRef]
    [Google Scholar]
  2. Bönike, R. & Juhasz, S. E. ( 1964; ). Beschreibung der neuen spezies Mycobacterium vaccae n. sp. Zentralbl Bakteriol Parasitenkd Infekt Hyg Abt I 192, 133–135.
    [Google Scholar]
  3. Butler, W. R. & Guthertz, L. S. ( 2001; ). Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14, 704–726.[CrossRef]
    [Google Scholar]
  4. Butler, W. R., Floyd, M. M., Silcox, M. S. & 9 other authors ( 1996; ). Standardized Method for HPLC Identification of Mycobacteria. Atlanta, GA: CDC, US Department of Health and Human Services.
  5. De Soete, G. ( 1983; ). A least square algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  6. DSMZ ( 2001; ). Catalogue of Strains, 7th edn. Braunschweig: DSMZ.
  7. Felsenstein, J. ( 1993; ). phylip (phylogenetic inference package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA.
  8. Garcia-Martinez, J., Acinas, S. G., Anton, A. I. & Rodriguez-Valera, F. ( 1999; ). Use of the 16S–23S ribosomal genes spacer region in studies of prokaryotic diversity. J Microbiol Methods 36, 55–64.[CrossRef]
    [Google Scholar]
  9. Gürtler, V. & Stanisich, V. A. ( 1996; ). New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142, 2–16.[CrossRef]
    [Google Scholar]
  10. Häggblom, M. M., Nohynek, L. J., Palleroni, N. J., Kronqvist, K., Nurmiaho-Lassila, E.-L., Salkinoja-Salonen, M. S., Klatte, S. & Kroppenstedt, R. M. ( 1994; ). Transfer of polychlorophenol-degrading Rhodococcus chlorophenolicus (Apajalathi et al. 1986) to the genus Mycobacterium as Mycobacterium chlorophenolicum comb. nov. Int J Syst Bacteriol 44, 485–493.[CrossRef]
    [Google Scholar]
  11. Hinrikson, H. P. & Pfyffer, G. E. ( 1994; ). Mycobacterial mycolic acids. Med Microbiol Lett 3, 49–57, 97–106.
    [Google Scholar]
  12. Jensen, M. A., Webster, J. A. & Straus, N. ( 1993; ). Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol 59, 945–952.
    [Google Scholar]
  13. Khan, A. A., Kim, S.-J., Paine, D. D. & Cerniglia, C. E. ( 2002; ). Classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, Mycobacterium sp. strain PYR-1, as Mycobacterium vanbaalenii sp. nov. Int J Syst Evol Microbiol 52, 1997–2002.[CrossRef]
    [Google Scholar]
  14. Kubica, G. P. & Pool, G. L. ( 1960; ). Studies on the catalase activity of acid-fast bacilli. I. An attempt to subgroup these organisms on the basis of their catalase activities at different temperatures and pH. Am Rev Respir Dis 81, 387–391.
    [Google Scholar]
  15. Maidak, B. L., Cole, J. R., Parker, C. T., Jr & 11 other authors ( 1999; ). A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27, 171–173.[CrossRef]
    [Google Scholar]
  16. Miller, J. L. ( 1997; ). Sherlock Mycobacteria Identification by High Performance Liquid Chromatography. A Training Manual. Newark, DE: MIDI Inc.
  17. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  18. Ranjard, L., Poly, F., Combrisson, J., Richaume, A., Gourbiere, F., Thioulouse, J. & Nazaret, S. ( 2000; ). Heterogeneous cell density and genetic structure of bacterial pools associated with various soil microenvironments as determined by enumeration and DNA fingerprinting approach (RISA). Microb Ecol 39, 263–272.
    [Google Scholar]
  19. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  20. Schröder, K.-H., Naumann, L., Kroppenstedt, R. M. & Reischl, U. ( 1997; ). Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium. Int J Syst Bacteriol 47, 86–91.[CrossRef]
    [Google Scholar]
  21. Schwiening, S. ( 1999; ). Mikrobieller Abbau von 14C-markiertem Benzo[a]pyren durch PAK-adaptierte Bakterienmischkulturen. PhD thesis, Aachen University. Aachen: Shaker Verlag.
  22. Sigler, W. V., Crivii, S. & Zeyer, J. ( 2002; ). Bacterial succession in glacial forefield soils characterized by community structure, activity, and opportunistic growth dynamics. Microb Ecol 44, 306–316.[CrossRef]
    [Google Scholar]
  23. Silcox, V. A., Good, R. C. & Floyd, M. M. ( 1981; ). Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol 14, 686–691.
    [Google Scholar]
  24. Sonderkamp, S., Vomberg, A., Schmitz, C., Faßbender, U. & Klinner, U. ( 2001; ). Interactions between bacterial populations during degradation of a lubricant base oil. FEMS Microbiol Ecol 38, 97–104.[CrossRef]
    [Google Scholar]
  25. Springer, B., Tortoli, E., Richter, I. & 7 other authors ( 1995; ). Mycobacterium conspicuum sp. nov., a new species isolated from patients with disseminated infections. J Clin Microbiol 33, 2805–2811.
    [Google Scholar]
  26. Stackebrandt, E., Rainey, F. & Ward-Rainey, N. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  27. Tsukamura, M. ( 1966; ). Adansonian classification of mycobacteria. J Gen Microbiol 45, 253–273.[CrossRef]
    [Google Scholar]
  28. Tsukamura, M., van der Meulen, H. J. & Grabow, W. O. K. ( 1983; ). Numerical taxonomy of rapidly growing, scotochromogenic mycobacteria of the Mycobacterium parafortuitum complex: Mycobacterium austroafricanum sp. nov. and Mycobacterium diernhoferi sp. nov., nom rev. Int J Syst Bacteriol 33, 460–469.[CrossRef]
    [Google Scholar]
  29. Vincent Lévy-Frébault, V. & Portaels, F. ( 1992; ). Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42, 315–323.[CrossRef]
    [Google Scholar]
  30. Wayne, L. G., Engbaek, H. C., Engel, H. W. B. & 17 other authors ( 1974; ). Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium: tests for pigment, urease, resistance to sodium chloride, hydrolysis of Tween 80, and β-galactosidase. Int J Syst Bacteriol 24, 412–419.[CrossRef]
    [Google Scholar]
  31. Williumsen, P. A. & Karlson, U. ( 1997; ). Screening of bacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation 7, 415–423.[CrossRef]
    [Google Scholar]
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