1887

Abstract

A novel rapidly growing, non-pigmented mycobacterium was isolated from blood samples obtained from 17 patients with febrile syndrome. Bacterial growth occurred at 30 and 37 °C on Löwenstein–Jensen medium and also on MacConkey agar without crystal violet. Strains contained - and ′-mycolates in their cell wall. Sequence analysis of the and 16S rRNA genes identified the isolates as rapidly growing mycobacteria. Sequences of both genes were unique within the mycobacteria. DNA–DNA hybridization showed that the isolates had less than 15 % reassociation with 13 other recognized rapidly growing mycobacteria. The name sp. nov. is proposed for this novel opportunistic pathogen, which is most closely related to . The type strain is 502329 (=CIP 107998=CCUG 47953).

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2004-09-01
2019-12-14
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vol. , part 5, pp. 1729–1734

Some characteristics of strain 502329 (Table A), TLC of methyl mycolates of strains and related species (Fig. A), GC analysis of fatty acid methyl esters of strain 502329 (Fig. B), hybridization dot-blot assays (Fig. C) and DNA–DNA relatedness values between strain 502329 and other species (Table B) are available to download. [PDF](358KB)



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