%0 Journal Article %A Yang, Ling-Ling %A Ao, Tao %A Wang, Xing-Hong %A He, Jie %A Klenk, Hans-Peter %A Tang, Shu-Kun %A Li, Wen-Jun %T Proposal of Intrasporangium mesophilum sp. nov., and reclassification of Humihabitans oryzae Kageyama et al. 2007 as Intrasporangium oryzae comb. nov. %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_5 %P 1037-1041 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.029934-0 %I Microbiology Society, %X A Gram-positive and aerobic bacterium, designated strain YIM 49065T, was isolated from rhizospheric soil of Jatropha curcas in Yunnan, China. This isolate formed branched and fragmented mycelia containing ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The major cellular fatty acid profile was characterized by iso-C14 : 0, iso-C15 : 0 and iso-C16 : 0, and the predominant menaquinone was MK-8(H4). The DNA G+C content was 69.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequence demonstrated that strain YIM 49065T was closely associated with Intrasporangium calvum DSM 43043T and Humihabitans oryzae KV-657T, exhibiting 98.8 % and 98.6 % 16S rRNA gene sequence similarities, respectively. Furthermore, DNA–DNA hybridizations among strains YIM 49065T, I. calvum DSM 43043T and H. oryzae DSM 22331T indicated that this isolate represented a novel species in the genus Intrasporangium . On the basis of these data, we proposed one novel species, Intrasporangium mesophilum sp. nov., for strain YIM 49065T ( = DSM 23217T  = CCTCC AA 209077T). Additionally, the comparison of biochemical and phylogenetic characters supported the reclassification of Humihabitans oryzae as a separate species within the genus Intrasporangium , Intrasporangium oryzae comb. nov. (type strain KV-657T  = JCM 15924T  = NBRC 101802T  = NRRL B-24470T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.029934-0