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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 12

sp. nov., isolated from a sandy beach, and emended description of the genus Free

Abstract

A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201, was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201 was observed between 15 and 45 °C (optimum, 30 °C) and between pH 5.0 and 9.0 (optimum, pH 6.0–7.5). Strain B201 contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201 were C cyclo, C, summed feature 2 (iso-C I/C 3-OH and/or C ALDE), C and C cyclo ω8. The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with DCY25 within the genus . Strain B201 was most closely related to DCY25 and MJ07 with similarities of 98.6 and 97.5 %, respectively. However, DNA–DNA relatedness values of strain B201 with DCY25 and MJ07 were 30.2±5.4 and 4.9±1.8 %, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is B201 ( = KACC 14927 = JCM 16917). An emended description of the genus is given.

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Funding
This study was supported by the:
  • Technology Development Program for Agriculture and Forestry (TDPAF) of the Ministry for Agriculture, Forestry and Fisheries
  • National Research Foundation of Korea (Award 2009-0071880)
© 2011 IUMS
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2011-12-01
2025-03-24
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Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus Pusillimonas
Int J Syst Evol Microbiol 61, 2901 (2011); https://doi.org/10.1099/ijs.0.029892-0
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