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Abstract

A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201, was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201 was observed between 15 and 45 °C (optimum, 30 °C) and between pH 5.0 and 9.0 (optimum, pH 6.0–7.5). Strain B201 contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201 were C cyclo, C, summed feature 2 (iso-C I/C 3-OH and/or C ALDE), C and C cyclo ω8. The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with DCY25 within the genus . Strain B201 was most closely related to DCY25 and MJ07 with similarities of 98.6 and 97.5 %, respectively. However, DNA–DNA relatedness values of strain B201 with DCY25 and MJ07 were 30.2±5.4 and 4.9±1.8 %, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is B201 ( = KACC 14927 = JCM 16917). An emended description of the genus is given.

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2011-12-01
2020-01-26
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Supplements

vol. , part 12, pp. 2901 - 2906

Maximum-parsimony (a) and maximum-likelihood (b) trees based on 16S rRNA gene sequences showing phylogenetic relationships of strain B201 and other related taxa.

Transmission electron micrograph of strain B201 .

A polar lipid TLC sprayed with molybdatophosphoric acid reagent of strain B201 following separation by 2D TLC.

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