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Abstract

A non-sporulating, non-motile, catalase- and oxidase-positive, Gram-negative, rod-shaped bacterial strain, designated BA-3, was isolated from activated sludge of a wastewater treatment facility. The strain was able to degrade about 95 % of 100 mg 3-phenoxybenzoic acid l within 2 days of incubation. Growth occurred in the presence of 0–2 % (w/v) NaCl [optimum, 0.5 % (w/v) NaCl], at pH 5.5–9.0 (optimum, pH 7.0) and at 10–37 °C (optimum, 28 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BA-3 was a member of the genus ; it showed highest gene sequence similarity to X23 (98.2 %), and similarities of <97.0 % with strains of other species. The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of summed feature 8 (Cω6 and/or Cω7) in the cellular fatty acid profile and the DNA G+C content also supported affiliation of the isolate to the genus . Strain BA-3 showed low DNA–DNA relatedness values (21.3±0.8 %) with X23. Based on phenotypic, genotypic and phylogenetic data, strain BA-3 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is BA-3 ( = CCTCC AB 2010217 = KCTC 23196 = KACC 16433).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970099)
  • Transformation Fund for Agricultural Science and Technology Achievements (Award 2009GB23600516)
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2012-04-01
2024-03-29
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